IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIPK2 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit HIPK2 Polyclonal Antibody | anti-HIPK2 antibody
HIPK2 Antibody (N-term D69)
Gene Names
HIPK2; PRO0593
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HIPK2, Antibody; HIPK2 Antibody (N-term D69); Homeodomain-interacting protein kinase 2; hHIPk2; HIPK2; anti-HIPK2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-468 amino acids from the N-terminal region of human HIPK2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
437-468
Sequence Length
1,171
Applicable Applications for anti-HIPK2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIPK2 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIPK2 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-HIPK2 Antibody (N-term D69) in Ramos cell line lysates (35ug/lane). HIPK2(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-HIPK2 Antibody (N-term D69) in Ramos cell line lysates (35ug/lane). HIPK2(arrow) was detected using the purified Pab.)
Related Product Information for anti-HIPK2 antibody
HIPK2, a member of the KIPK subfamily of Ser/Thr protein kinases, phosphorylates homeodomain transcription factors and may play a role as a corepressor for homeodomain transcription factors. This nuclear protein has been shown to interact with TRADD. It is highly expressed in neuronal tissues, heart and kidney, and weakly expressed in a ubiquitous way. HIPK2 is a target for sumoylation, and when conjugated it is directed to nuclear speckles.
Product Categories/Family for anti-HIPK2 antibody
References
Li, X., et al., Biochem. Biophys. Res. Commun. 277(2):513-517 (2000).
Wang, Y., et al., Biochim. Biophys. Acta 1518 (1-2), 168-172 (2001).
Wang, Y., et al., Biochim. Biophys. Acta 1518 (1-2), 168-172 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130966
NCBI Official Full Name
homeodomain-interacting protein kinase 2 isoform 2
NCBI Official Synonym Full Names
homeodomain interacting protein kinase 2
NCBI Official Symbol
HIPK2
NCBI Official Synonym Symbols
PRO0593
NCBI Protein Information
homeodomain-interacting protein kinase 2
UniProt Protein Name
Homeodomain-interacting protein kinase 2
UniProt Gene Name
HIPK2
UniProt Synonym Gene Names
hHIPk2
UniProt Entry Name
HIPK2_HUMAN
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Product Notes
The HIPK2 hipk2 (Catalog #AAA286621) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 437-468. The HIPK2 Antibody (N-term D69) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's HIPK2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HIPK2 hipk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIPK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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