Rabbit Phospho-HIPK2 (Y361) Polyclonal Antibody | anti-HIPK2 antibody

Phospho-HIPK2 (Y361) Antibody

Cat. Num. AAA288429

• Gene Names: HIPK2; PRO0593
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Applications: ELISA, Dot Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: Phospho-HIPK2 (Y361), Antibody; Phospho-HIPK2 (Y361) Antibody; Homeodomain-interacting protein kinase 2; hHIPk2; HIPK2; anti-HIPK2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This HIPK2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y361 of human HIPK2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 1,171

• Applicable Applications for anti-HIPK2 antibody: ELISA, DB (Dot Blot)
• Function: Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53- dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. Modulates HMGA1 DNA-binding affinity. In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.
• Cellular Location: Nucleus, PML body. Cytoplasm. Note=Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic
• Tissue Location: Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

DB (Dot Blot)

(Dot blot analysis of anti-Phospho-HIPK2-pY361 Antibody (AAA288429) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-HIPK2 antibody

HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.

Product Categories/Family for anti-HIPK2 antibody

Cell Biology; Signal Transduction; Phospho Antibodies

References

Guha U, et al. (2008) Proc Natl Acad Sci U S A 105, 14112-7
Daub H, et al. (2008) Mol Cell 31, 438-48
Guo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7
Rikova K, et al. (2007) Cell 131, 1190-203

NCBI and Uniprot Product Information

• NCBI GI #: 164420691
• NCBI GeneID: 28996
• NCBI Accession #: NP_001106710.1; NP_073577.3
• NCBI GenBank Nucleotide #: NM_001113239.2
• UniProt Accession #: Q9H2X6; Q75MR7; Q8WWI4; Q9H2Y1
• Molecular Weight: Calculated MW: 130966
• NCBI Official Full Name: homeodomain-interacting protein kinase 2 isoform 2
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 2
• NCBI Official Symbol: HIPK2
• NCBI Official Synonym Symbols: PRO0593
• NCBI Protein Information: homeodomain-interacting protein kinase 2
• UniProt Protein Name: Homeodomain-interacting protein kinase 2
• UniProt Gene Name: HIPK2
• UniProt Synonym Gene Names: hHIPk2
• UniProt Entry Name: HIPK2_HUMAN