Rabbit Histone H2A.Z Polyclonal Antibody | anti-H2A.Z antibody

Histone H2A.Z antibody (pAb)(H2AZ)

Cat. Num. AAA59953

• Gene Names: H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
• Reactivity: Human, Wide Range Predicted
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Protein A Chromatography
• Synonyms: Histone H2A.Z, Antibody; Histone H2A.Z antibody (pAb)(H2AZ); anti-H2A.Z antibody

• Host: Rabbit
• Reactivity: Human, Wide Range Predicted
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A Chromatography
• Form/Format: Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version of this antibody is also available.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-H2A.Z antibody: WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
• Immunogen: This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

DB (Dot Blot)

(Histone H2A.Z antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A.Z antibody for Histone H2A.Z. Recombinant proteins were spotted onto PVDF and probed with Histone H2A.Z antibody at a 1 ug/ml dilution. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Recombinant H2A. Lane 2: Recombinant H2A.Z. Lane 3: Recombinant H4.)

WB (Western Blot)

(Histone H2A.Z antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (25 ?g) probed with Histone H2A.Z antibody (pAb) (1 ug/ml). Specificity was verified by peptide competition. Lane 1: no peptide. Lane 2: 1?m immunizing peptide.)

IF (Immunofluorescence)

(Histone H2A.Z antibody (pAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with Histone H2A.Z antibody at a 0.5 ug/ml dilution.)

ChIP (Chromatin Immunoprecipitation)

(Histone H2A.Z antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Histone H2A.Z pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)

ChIP (Chromatin Immunoprecipitation)

(Histone H2A.Z antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ?g of chromatin from a human medulloblastoma cell line and 4 ?l of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 18 million sequence tags were mapped to identify H2A.Z occupancy. H2A.Z is found throughout the genome, is often enriched at promoters and depleted from transcribed genes as shown in the images.)

Related Product Information for anti-H2A.Z antibody

Short Description: Our Histone H2A.Z antibody (pAb)(H2AZ) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may enable H2A.Z to function as an insulator of chromatin domains.

Product Categories/Family for anti-H2A.Z antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4504255
• NCBI GeneID: 3015
• NCBI Accession #: NP_002097; AB_2793401
• NCBI GenBank Nucleotide #: NM_002106.3
• UniProt Accession #: P0C0S5; P17317; Q6I9U0; B2RD56
• Molecular Weight: 15.9 kDa (151aa)
• NCBI Official Full Name: histone H2A.Z
• NCBI Official Synonym Full Names: H2A histone family member Z
• NCBI Official Symbol: H2AFZ
• NCBI Official Synonym Symbols: H2AZ; H2A.z; H2A/z; H2A.Z-1
• NCBI Protein Information: histone H2A.Z
• UniProt Protein Name: Histone H2A.Z
• UniProt Gene Name: H2AFZ
• UniProt Synonym Gene Names: H2AZ; H2A/z
• UniProt Entry Name: H2AZ_HUMAN