DB (Dot Blot)
(Histone H2B acetyl Lys46 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys46 pAb for acetyl Lys46 histone H2B. Decreasing amounts of acetylated peptides corresponding to the immunogen and related sequences were spotted onto PVDF and probed with the antibody at 1:4,000. Lane 1: acetyl Lys46 histone H2B peptide. Lane 2: unmodified Lys46 histone H2B peptide. No detection of peptides (acetylated) corresponding to lysine 9, 14, 18, 23, 27, and 56 of Histone H3 was observed with Histone H2B acetyl Lys46 pAb. In addition, no detection of peptides (acetylated) corresponding to lysine 5, 15, 16, and 120 of Histone H2B was observed with Histone H2B acetyl Lys46 pAb.)
Rabbit Histone H2BK46ac Polyclonal Antibody | anti-H2BK46ac antibody
Histone H2BK46ac antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Synonyms
Histone H2BK46ac, Antibody; Histone H2BK46ac antibody (pAb); anti-H2BK46ac antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H2BK46ac antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation)
Conjugation
Acetylated
Immunogen
This Histone H2B acetyl Lys46 antibody was raised against a peptide containing acetyl-lysine 46 of human histone H2B.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Histone H2B acetyl Lys46 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys46 pAb for acetyl Lys46 histone H2B. Decreasing amounts of acetylated peptides corresponding to the immunogen and related sequences were spotted onto PVDF and probed with the antibody at 1:4,000. Lane 1: acetyl Lys46 histone H2B peptide. Lane 2: unmodified Lys46 histone H2B peptide. No detection of peptides (acetylated) corresponding to lysine 9, 14, 18, 23, 27, and 56 of Histone H3 was observed with Histone H2B acetyl Lys46 pAb. In addition, no detection of peptides (acetylated) corresponding to lysine 5, 15, 16, and 120 of Histone H2B was observed with Histone H2B acetyl Lys46 pAb.)
DB (Dot Blot)
(Histone H2B acetyl Lys46 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys46 pAb for acetyl Lys46 histone H2B. Decreasing amounts of acetylated peptides corresponding to the immunogen and related sequences were spotted onto PVDF and probed with the antibody at 1:4,000. Lane 1: acetyl Lys46 histone H2B peptide. Lane 2: unmodified Lys46 histone H2B peptide. No detection of peptides (acetylated) corresponding to lysine 9, 14, 18, 23, 27, and 56 of Histone H3 was observed with Histone H2B acetyl Lys46 pAb. In addition, no detection of peptides (acetylated) corresponding to lysine 5, 15, 16, and 120 of Histone H2B was observed with Histone H2B acetyl Lys46 pAb.)
WB (Western Blot)
(Histone H2B acetyl Lys46 pAb tested by Western blot. Acid extract of HeLa cells (20 ug per lane) was probed with Histone H2B acetyl Lys46 polyclonal antibody (1:4,000 dilution). Lane 1: Untreated cells. Lane 2: Cells treated with sodium butyrate. Lane 3: Recombinant Histone H2B (200 ng).)
WB (Western Blot)
(Histone H2B acetyl Lys46 pAb tested by Western blot. Acid extract of HeLa cells (20 ug per lane) was probed with Histone H2B acetyl Lys46 polyclonal antibody (1:4,000 dilution). Lane 1: Untreated cells. Lane 2: Cells treated with sodium butyrate. Lane 3: Recombinant Histone H2B (200 ng).)
ChIP (Chromatin Immunoprecipitation)
(Histone H2B acetyl Lys46 pAb tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and 50 ul of Ready-to-ChIP HeLa Chromatin per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR. Lane 1: ChIP using negative control rabbit IgG. Lane 2: ChIP using 10 ul of Histone H2B acetyl Lys46 pAb. Lane 3: PCR input control.)
ChIP (Chromatin Immunoprecipitation)
(Histone H2B acetyl Lys46 pAb tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and 50 ul of Ready-to-ChIP HeLa Chromatin per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR. Lane 1: ChIP using negative control rabbit IgG. Lane 2: ChIP using 10 ul of Histone H2B acetyl Lys46 pAb. Lane 3: PCR input control.)
Related Product Information for anti-H2BK46ac antibody
Short Description: Our Histone H2BK46ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.
Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.
Product Categories/Family for anti-H2BK46ac antibody
NCBI and Uniprot Product Information
Molecular Weight
15kDa
Similar Products
Product Notes
The H2BK46ac (Catalog #AAA59884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2BK46ac antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2BK46ac can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H2BK46ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2BK46ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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