WB (Western Blot)
(Western blot of Histone H3.3K27M pAb. H1299 cell extracts transfected with human Histone H3.3 wild-type (wt) or various Histone H3.3 single amino acid substitutions were probed with Histone H3.3K27M antibody (pAb) at 2 ug/ml. Beta-actin was used as a loading control.)
Rabbit anti-Human Histone H3.3K27M Polyclonal Antibody | anti-H3.3K27M antibody
Histone H3.3K27M antibody (pAb)
Gene Names
H3F3A; H3F3; H3.3A
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Histone H3.3K27M, Antibody; Histone H3.3K27M antibody (pAb); anti-H3.3K27M antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H3.3K27M antibody
WB (Western Blot)
Immunogen
This antibody was generated using a Peptide containing a Methionine substitution at lysine 27 of human Histone H3.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Western blot of Histone H3.3K27M pAb. H1299 cell extracts transfected with human Histone H3.3 wild-type (wt) or various Histone H3.3 single amino acid substitutions were probed with Histone H3.3K27M antibody (pAb) at 2 ug/ml. Beta-actin was used as a loading control.)
WB (Western Blot)
(Western blot of Histone H3.3K27M pAb. H1299 cell extracts transfected with human Histone H3.3 wild-type (wt) or various Histone H3.3 single amino acid substitutions were probed with Histone H3.3K27M antibody (pAb) at 2 ug/ml. Beta-actin was used as a loading control.)
WB (Western Blot)
(Western blot analysis of Hantibody specificity for Histone H3.3K27M 0.1 ug per lane of Recombinant Human Histone H3.3 (Lanes 1 & 3) or Recombinant Human Histone H3.3K27M (Lanes 2 & 4) were probed with AbFlex Histone H3.3 antibody (rAb)) or Histone H3.3K27M antibody (pAb) at 0.05 ug/ml.)
WB (Western Blot)
(Western blot analysis of Hantibody specificity for Histone H3.3K27M 0.1 ug per lane of Recombinant Human Histone H3.3 (Lanes 1 & 3) or Recombinant Human Histone H3.3K27M (Lanes 2 & 4) were probed with AbFlex Histone H3.3 antibody (rAb)) or Histone H3.3K27M antibody (pAb) at 0.05 ug/ml.)
Related Product Information for anti-H3.3K27M antibody
Short Description: Our Histone H3.3K27M antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Histone H3.3 point mutations (K27 and G34) are present in 1/3 of pediatric glioblastomas. Up to 78% of diffuse intrinsic pontine gliomas (DIPGs) carries K27M and 36% of non-brainstem gliomas carries either K27M or G34R/V mutations. H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Histone H3.3 point mutations (K27 and G34) are present in 1/3 of pediatric glioblastomas. Up to 78% of diffuse intrinsic pontine gliomas (DIPGs) carries K27M and 36% of non-brainstem gliomas carries either K27M or G34R/V mutations. H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation.
Product Categories/Family for anti-H3.3K27M antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.7kDa (159aa)
NCBI Official Full Name
histone H3.3
NCBI Official Synonym Full Names
H3 histone family member 3A
NCBI Official Symbol
H3F3A
NCBI Official Synonym Symbols
H3F3; H3.3A
NCBI Protein Information
histone H3.3
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3F3A
UniProt Synonym Gene Names
H3.3A; H3F3
UniProt Entry Name
H33_HUMAN
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Product Notes
The H3.3K27M h3f3a (Catalog #AAA60091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3.3K27M antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3.3K27M can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the H3.3K27M h3f3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3.3K27M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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