Application Data
(Histone H4R3me2a (asymmetric) antibody (pAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4R3me2a antibody was applied at a dilution of 1:20,000 to MODified Histone Peptide Array . The arrays were scanned with ArrayAnalysis Software 16 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.)
Rabbit Histone H4R3me2a Polyclonal Antibody | anti-H4R3me2a antibody
Histone H4R3me2a (asymmetric) antibody (pAb)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
Histone H4R3me2a, Antibody; Histone H4R3me2a (asymmetric) antibody (pAb); anti-H4R3me2a antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H4R3me2a antibody
WB (Western Blot), IF (Immunofluorescence), DB (Dot Blot), ChIP (Chromatin immunoprecipitation)
Conjugation
Methylated
Immunogen
This Histone H4 dimethyl Arg3, asymmetric antibody was raised against a synthetic peptide containing dimethyl-Arg3 (asymmetric) of human histone H4.
Abs Residue
Arg
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Application Data
(Histone H4R3me2a (asymmetric) antibody (pAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4R3me2a antibody was applied at a dilution of 1:20,000 to MODified Histone Peptide Array . The arrays were scanned with ArrayAnalysis Software 16 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.)
Application Data
(Histone H4R3me2a (asymmetric) antibody (pAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4R3me2a antibody was applied at a dilution of 1:20,000 to MODified Histone Peptide Array . The arrays were scanned with ArrayAnalysis Software 16 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.)
DB (Dot Blot)
(Histone H4R3me2a (asymmetric) antibody (pAb)tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 asymmetric antibody for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 asymmetric antibody at 1:2500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H4R3me unmodified. Lane 2: H4R3me2a peptide. Lane 3: H4R3me2s peptide. Lane 4: H4R3me1 peptide.)
DB (Dot Blot)
(Histone H4R3me2a (asymmetric) antibody (pAb)tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 asymmetric antibody for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 asymmetric antibody at 1:2500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H4R3me unmodified. Lane 2: H4R3me2a peptide. Lane 3: H4R3me2s peptide. Lane 4: H4R3me1 peptide.)
WB (Western Blot)
(Histone H4R3me2a (asymmetric) antibody (pAb)tested by Western blot. Nuclear extract of HeLa cells (20 ug) tested by Western blot using Histone H4R3me2a (asymmetric) antibody (pAb) at a dilution of 1:1000.)
WB (Western Blot)
(Histone H4R3me2a (asymmetric) antibody (pAb)tested by Western blot. Nuclear extract of HeLa cells (20 ug) tested by Western blot using Histone H4R3me2a (asymmetric) antibody (pAb) at a dilution of 1:1000.)
Related Product Information for anti-H4R3me2a antibody
Short Description: Our Histone H4R3me2a (asymmetric) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Asymmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional activation by nuclear hormone receptors. Arg3 methylation has been reported to facilitate the subsequent acetylation of histone H4 by the acetyltransferase p300. Histone H4 dimethyl Arg3 asymmetric methylation (H4R2me2a) is catalyzed by PRMT1 and PRMT6.
Background: Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Asymmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional activation by nuclear hormone receptors. Arg3 methylation has been reported to facilitate the subsequent acetylation of histone H4 by the acetyltransferase p300. Histone H4 dimethyl Arg3 asymmetric methylation (H4R2me2a) is catalyzed by PRMT1 and PRMT6.
Product Categories/Family for anti-H4R3me2a antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,367 Da
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 4, H4
NCBI Official Symbol
HIST4H4
NCBI Official Synonym Symbols
H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
NCBI Protein Information
histone H4; histone 4, H4; OTTHUMP00000239323
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
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Product Notes
The H4R3me2a hist1h4a (Catalog #AAA59792) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H4R3me2a (asymmetric) antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4R3me2a can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), DB (Dot Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H4R3me2a hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4R3me2a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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