IF (Immunofluorescence)
(Immunofluorescence analysis of 293T cell using H4R3me2a antibody. Blue: DAPI for nuclear staining.)
Rabbit Histone H4R3me2a Polyclonal Antibody | anti-HIST3H3 antibody
Histone H4R3me2a Antibody
Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Immunofluorescence, Western Blot
Purity
Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.
Synonyms
Histone H4R3me2a, Antibody; Histone H4R3me2a Antibody; H3t; H3.4; H3/g; H3FT; anti-HIST3H3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.
Form/Format
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
136
Applicable Applications for anti-HIST3H3 antibody
ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
Modification
R3me2a
Target Name
Histone H4
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of 293T cell using H4R3me2a antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of 293T cell using H4R3me2a antibody. Blue: DAPI for nuclear staining.)
DB (Dot Blot)
(Dot-blot analysis of all sorts of methylation peptidesusing H4R3 me2a antibody.)
DB (Dot Blot)
(Dot-blot analysis of all sorts of methylation peptidesusing H4R3 me2a antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E Coli., using H4R3 me2a antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E Coli., using H4R3 me2a antibody.)
Related Product Information for anti-HIST3H3 antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Product Categories/Family for anti-HIST3H3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,508 Da
NCBI Official Full Name
histone H3.1t
UniProt Protein Name
Histone H3.1t
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
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Product Notes
The Histone H4R3me2a hist3h3 (Catalog #AAA301107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H4R3me2a Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4R3me2a can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Histone H4R3me2a hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4R3me2a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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