WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: A549) using HLA DRB3 antibody at a dilution of 1:1000)
Rabbit HLA DRB3 Polyclonal Antibody | anti-HLA DRB3 antibody
HLA DRB3 antibody
Gene Names
HLA-DRB3; SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B; HLA-DR3B
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
HLA DRB3 antibody was purified by antigen-affinity chromatography
Synonyms
HLA DRB3, Antibody; HLA DRB3 antibody; Polyclonal HLA DRB3; Anti-HLA DRB3; MHC class II antigen DR beta 3 chain; HLA class II histocompatibility antigen DR beta 3 chain; HLA-DRB1; HLA DRB3; HLA DRB-3; MHC class II antigen DRB3; HLA-DR3B; MGC117330; MHC class II HLA-DR beta 3 chain; HLA DRB 3; HLA-DRB3; anti-HLA DRB3 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
HLA DRB3 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
81
Applicable Applications for anti-HLA DRB3 antibody
WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Biological Significance
HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
Cross-Reactivity
Human
Immunogen
HLA DRB3 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 4 and 266 of HLA-DRB3
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: A549) using HLA DRB3 antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: A549) using HLA DRB3 antibody at a dilution of 1:1000)
IF (Immunofluorescence)
(Immunofluorescence analysis of methanol-fixed A549, using HLA-DRB3 antibody at 1:500 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of methanol-fixed A549, using HLA-DRB3 antibody at 1:500 dilution.)
Related Product Information for anti-HLA DRB3 antibody
Affinity purified Rabbit polyclonal HLA DRB3 antibody
Product Categories/Family for anti-HLA DRB3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
30 kDa
NCBI Official Full Name
HLA-DRB3, partial
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DR beta 3
NCBI Official Symbol
HLA-DRB3
NCBI Official Synonym Symbols
SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B; HLA-DR3B
NCBI Protein Information
major histocompatibility complex, class II, DR beta 3
UniProt Protein Name
HLA class II histocompatibility antigen, DR beta 3 chain
UniProt Gene Name
HLA-DRB3
UniProt Entry Name
DRB3_HUMAN
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Product Notes
The HLA DRB3 hla-drb3 (Catalog #AAA107876) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HLA DRB3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the HLA DRB3 hla-drb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA DRB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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