IP (Immunoprecipitation)
(HMGB3 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-HMGB3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HMGB3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :23 kDa)
Rabbit anti-Human HMG4/HMGB3 Polyclonal Antibody | anti-HMG4/HMGB3 antibody
Anti-HMG4/HMGB3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
HMG4/HMGB3, Antibody; Anti-HMG4/HMGB3 Antibody, Rabbit Polyclonal; high mobility group box 3; Anti-HMG-2a Antibody; Anti-HMG-4 Antibody; Anti-HMG2A Antibody; Anti-HMG4 Antibody; anti-HMG4/HMGB3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human HMG4/HMGB3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-HMG4/HMGB3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human HMG4/HMGB3
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HMG4/HMGB3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(HMGB3 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-HMGB3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HMGB3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :23 kDa)
IP (Immunoprecipitation)
(HMGB3 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-HMGB3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HMGB3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :23 kDa)
WB (Western Blot)
(Anti-HMG4/HMGB3 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa)
WB (Western Blot)
(Anti-HMG4/HMGB3 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa)
Related Product Information for anti-HMG4/HMGB3 antibody
High mobility group box protein 3 (HMGB3) regulates proliferation and inflammatory response in vertebrates. High mobility group box 3 (HMGB3), a member of the high-mobility group box (HMGB) family, was reported to be over-expressed in gastric carcinoma and bladder cancer, affect tumor initiation and progression. HMGB3 may serve as an oncoprotein and could be used as a potential prognostic marker in colorectal cancer (CRC). HMBG3 may be a potential molecular marker for predicting the prognosis of esophageal squamous cell carcinoma (ESCC) patients. HMGB3 may serve as an important oncoprotein and indicate that overexpression of HMGB3 in Human urinary bladder cancer (UBC) could be used as a potential prognostic marker.
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Product Notes
The HMG4/HMGB3 (Catalog #AAA258755) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HMG4/HMGB3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMG4/HMGB3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HMG4/HMGB3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMG4/HMGB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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