FCM/FACS (Flow Cytometry)
(HMOX1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human HMOX1 Polyclonal Antibody | anti-HMOX1 antibody
HMOX1 Antibody (Center)
Gene Names
HMOX1; HO-1; HSP32; HMOX1D; bK286B10
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HMOX1, Antibody; HMOX1 Antibody (Center); Heme oxygenase 1; HO-1; HMOX1; HO; HO1; anti-HMOX1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HMOX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-204 amino acids from the Central region of human HMOX1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
177-204
Sequence Length
288
Applicable Applications for anti-HMOX1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HMOX1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HMOX1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of HMOX1 (arrow) using rabbit polyclonal HMOX1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMOX1 gene.)
WB (Western Blot)
(Western blot analysis of HMOX1 (arrow) using rabbit polyclonal HMOX1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMOX1 gene.)
Related Product Information for anti-HMOX1 antibody
Heme oxygenase, an essential enzyme in heme catabolism,
cleaves heme to form biliverdin, which is subsequently converted to
bilirubin by biliverdin reductase, and carbon monoxide, a putative
neurotransmitter. Heme oxygenase activity is induced by its
substrate heme and by various nonheme substances. Heme oxygenase
occurs as 2 isozymes, an inducible heme oxygenase-1 and a
constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme
oxygenase family.
cleaves heme to form biliverdin, which is subsequently converted to
bilirubin by biliverdin reductase, and carbon monoxide, a putative
neurotransmitter. Heme oxygenase activity is induced by its
substrate heme and by various nonheme substances. Heme oxygenase
occurs as 2 isozymes, an inducible heme oxygenase-1 and a
constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme
oxygenase family.
Product Categories/Family for anti-HMOX1 antibody
References
Wu, M.M., et al. Toxicol. Appl. Pharmacol. 248(3):226-233(2010)
Bolisetty, S., et al. J. Am. Soc. Nephrol. 21(10):1702-1712(2010)
Bao, W., et al. PLoS ONE 5 (8), E12371 (2010) :
Wu, M.M., et al. J. Biomed. Sci. 17, 70 (2010) :
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :
Bolisetty, S., et al. J. Am. Soc. Nephrol. 21(10):1702-1712(2010)
Bao, W., et al. PLoS ONE 5 (8), E12371 (2010) :
Wu, M.M., et al. J. Biomed. Sci. 17, 70 (2010) :
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32819
NCBI Official Full Name
heme oxygenase 1
NCBI Official Synonym Full Names
heme oxygenase 1
NCBI Official Symbol
HMOX1
NCBI Official Synonym Symbols
HO-1; HSP32; HMOX1D; bK286B10
NCBI Protein Information
heme oxygenase 1
UniProt Protein Name
Heme oxygenase 1
UniProt Gene Name
HMOX1
UniProt Synonym Gene Names
HO; HO1; HO-1
UniProt Entry Name
HMOX1_HUMAN
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Product Notes
The HMOX1 hmox1 (Catalog #AAA288535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 177-204. The HMOX1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMOX1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HMOX1 hmox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMOX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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