IP (Immunoprecipitation)
(Detection of human HNF1-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody AAA212480 (lot AAA212480-1) used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies)
Rabbit anti-Human HNF1-alpha Polyclonal Antibody | anti-HNF1-alpha antibody
Rabbit anti-HNF1-alpha Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
HNF1-alpha, Antibody; Rabbit anti-HNF1-alpha Antibody, Affinity Purified; HNF4A; transcription factor 1, hepatic; Transcription factor 1; TCF-1; TCF1; MODY3; liver-specific transcription factor LF-B1; truncated hepatocyte nuclear factor 1 alpha; LFB1; HNF-1-alpha; HNF-1A; HNF1; hepatocyte nuclear factor 1-alpha; hepatic nuclear factor 1; albumin proximal factor; interferon production regulator factor; IDDM20; Hepatocyte nuclear factor 1-alpha; HNF1 homeobox A; anti-HNF1-alpha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-HNF1-alpha antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 581 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human HNF1-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody AAA212480 (lot AAA212480-1) used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies)
IP (Immunoprecipitation)
(Detection of human HNF1-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody AAA212480 (lot AAA212480-1) used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies)
IHC (Immunohistochemistry)
(Detection of human HNF1-alpha by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HNF1-alpha (Cat. No. Lot1, Lot1, Lot1, and AAA212480 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human HNF1-alpha by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HNF1-alpha (Cat. No. Lot1, Lot1, Lot1, and AAA212480 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-HNF1-alpha antibody
Hepatocyte nuclear factor 1-alpha (HNF1-Alpha) is a transcription factor required for the expression of several liver-specific genes. HNF1-Alpha functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in the HNF1A gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas [taken from NCBI Entrez Gene (Gene ID: 6927)].
Product Categories/Family for anti-HNF1-alpha antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HNF1-alpha hnf1a (Catalog #AAA212480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HNF1-alpha Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HNF1-alpha can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the HNF1-alpha hnf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF1-alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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