WB (Western Blot)
(Western blot analysis of HO-2, pAb: Lane 1: MW marker, Lane 2: HO-2 Recombinant Human Protein, Lane 3: Human Liver Microsome Extract, Lane 4: Mouse Liver Microsome Extract, Lane 5: Rat Liver Microsome Extract, Lane 6: Dog Liver Microsome Extract.)
Rabbit HO-2 Polyclonal Antibody | anti-HO-2 antibody
HO-2 polyclonal antibody
Gene Names
HMOX2; HO-2
Reactivity
Human, Mouse, Rat, Dog, Pig, Monkey, Hamster
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Protein A affinity purified.
Synonyms
HO-2, Antibody; HO-2 polyclonal antibody; HMOX2; Hsp32; Heat shock protein 32; Heme oxygenase 2; HO-2, pAb; anti-HO-2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig, Monkey, Hamster
Clonality
Polyclonal
Specificity
Recognizes human, mouse, rat, dog, hamster, monkey and pig HO-2. Detects a band of ~36kDa by Western blot.
Purity/Purification
Protein A affinity purified.
Form/Format
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Sequence Length
316
Applicable Applications for anti-HO-2 antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA
Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of human HO-2.
Preparation and Storage
Long Term: -20 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of HO-2, pAb: Lane 1: MW marker, Lane 2: HO-2 Recombinant Human Protein, Lane 3: Human Liver Microsome Extract, Lane 4: Mouse Liver Microsome Extract, Lane 5: Rat Liver Microsome Extract, Lane 6: Dog Liver Microsome Extract.)
WB (Western Blot)
(Western blot analysis of HO-2, pAb: Lane 1: MW marker, Lane 2: HO-2 Recombinant Human Protein, Lane 3: Human Liver Microsome Extract, Lane 4: Mouse Liver Microsome Extract, Lane 5: Rat Liver Microsome Extract, Lane 6: Dog Liver Microsome Extract.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of human breast cancer tissue with HO-2 pAb.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of human breast cancer tissue with HO-2 pAb.)
IF (Immunofluorescence)
(Immunofluorescent analysis (confocal) staining of MCF-7 cells using HO-2 pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.)
IF (Immunofluorescence)
(Immunofluorescent analysis (confocal) staining of MCF-7 cells using HO-2 pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.)
Related Product Information for anti-HO-2 antibody
Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
Product Categories/Family for anti-HO-2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
32,837 Da
NCBI Official Full Name
Heme oxygenase 2
NCBI Official Synonym Full Names
heme oxygenase 2
NCBI Official Symbol
HMOX2
NCBI Official Synonym Symbols
HO-2
NCBI Protein Information
heme oxygenase 2
UniProt Protein Name
Heme oxygenase 2
UniProt Gene Name
HMOX2
UniProt Synonym Gene Names
HO2; HO-2
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Product Notes
The HO-2 hmox2 (Catalog #AAA77026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HO-2 polyclonal antibody reacts with Human, Mouse, Rat, Dog, Pig, Monkey, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's HO-2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA. Researchers should empirically determine the suitability of the HO-2 hmox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HO-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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