Rabbit HOOK3 Polyclonal Antibody | anti-HOOK3 antibody

HOOK3 Antibody

Cat. Num. AAA40853

• Gene Names: HOOK3; HK3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: HOOK3, Antibody; HOOK3 Antibody; Hook microtubule-tethering protein 3, HK3, h-hook3, hHK3, FLJ31058, Protein Hook homolog 3; anti-HOOK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 718

• Applicable Applications for anti-HOOK3 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: Rat Brain Tissue Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of HOOK3 in mouse brain tissue with HOOK3 antibody at 20 μg/ml.Red: HOOK3 Antibody (8425)Blue: DAPI staining)

IHC (Immunohiostchemistry)

(Immunohistochemistry of HOOK3 in mouse brain tissue with HOOK3 antibody at 10 μg/ml.)

WB (Western Blot)

(Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 μg/ml.)

Related Product Information for anti-HOOK3 antibody

The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems; their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).

Product Categories/Family for anti-HOOK3 antibody

Homeostasis

References

Walenta JH, Didier AJ, Liu X, et al. The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 2001; 152:923-34. Sano H, Ishino M, Krämer H, et al. The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. J. Biol. Chem. 2007; 282:7973-81. Herrmann L, Wiegmann C, Arsalan-Werner A, et al. Hook proteins: association with Alzheimer pathology and regulatory role of hook3 in amyloid beta generation. PLoS One 2015; 10:e0119423. Ciampi R, Giordano TJ, Wikenheiser-Brokamp K, et al. HOOK3-RET: a novel type of RET/PTC rearrangement in papillary thyroid carcinoma. Endocr. Relat. Cancer 2007; 14:445-52.

NCBI and Uniprot Product Information

• NCBI GI #: 14165274
• NCBI GeneID: 84376
• NCBI Accession #: NP_115786
• NCBI GenBank Nucleotide #: NM_032410.3
• UniProt Accession #: Q86VS8; Q8NBH0; Q9BY13; D3DSY8
• Molecular Weight: Predicted: 79 kDa; Observed: 77 kDa
• NCBI Official Full Name: protein Hook homolog 3
• NCBI Official Synonym Full Names: hook microtubule-tethering protein 3
• NCBI Official Symbol: HOOK3
• NCBI Official Synonym Symbols: HK3
• NCBI Protein Information: protein Hook homolog 3
• UniProt Protein Name: Protein Hook homolog 3
• UniProt Gene Name: HOOK3
• UniProt Synonym Gene Names: h-hook3; hHK3
• UniProt Entry Name: HOOK3_HUMAN