IP (Immunoprecipitation)
(Immunoprecipitating HOOK3 in 293 whole cell lysateLane 1: Rabbit control IgG instead of CSB-PA769790LA01HU in 293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-PA769790LA01HU (6ug) + 293 whole cell lysate (500ug)Lane 3: 293 whole cell lysate (20ug))
Rabbit anti-Human HOOK3 Polyclonal Antibody | anti-HOOK3 antibody
HOOK3 Antibody
Gene Names
HOOK3; HK3
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
>95%Protein G Purified
Synonyms
HOOK3, Antibody; HOOK3 Antibody; Protein Hook homolog 3; h-hook3; hHK3; HOOK3; anti-HOOK3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Protein G Purified
Form/Format
Liquid
Sequence Length
718
Applicable Applications for anti-HOOK3 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human Protein Hook homolog 3 protein (357-455AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating HOOK3 in 293 whole cell lysateLane 1: Rabbit control IgG instead of CSB-PA769790LA01HU in 293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-PA769790LA01HU (6ug) + 293 whole cell lysate (500ug)Lane 3: 293 whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating HOOK3 in 293 whole cell lysateLane 1: Rabbit control IgG instead of CSB-PA769790LA01HU in 293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-PA769790LA01HU (6ug) + 293 whole cell lysate (500ug)Lane 3: 293 whole cell lysate (20ug))
IHC (Immunohiostchemistry)
(IHC image of CSB-PA769790LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry)
(IHC image of CSB-PA769790LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: 293 whole cell lysateAll lanes: HOOK3 antibody at 4.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 84 kDaObserved band size: 84 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: 293 whole cell lysateAll lanes: HOOK3 antibody at 4.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 84 kDaObserved band size: 84 kDa)
Product Categories/Family for anti-HOOK3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,126 Da
NCBI Official Full Name
protein Hook homolog 3
NCBI Official Synonym Full Names
hook microtubule tethering protein 3
NCBI Official Symbol
HOOK3
NCBI Official Synonym Symbols
HK3
NCBI Protein Information
protein Hook homolog 3
UniProt Protein Name
Protein Hook homolog 3
UniProt Gene Name
HOOK3
UniProt Synonym Gene Names
h-hook3; hHK3
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Product Notes
The HOOK3 hook3 (Catalog #AAA235444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HOOK3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HOOK3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HOOK3 hook3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOOK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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