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WB (Western Blot)
(HP Antibody (Center) western blot analysis in MDA-MB453,CEM,NCI-H460,HepG2,K562 cell line lysates (35ug/lane).This demonstrates the HP antibody detected the HP protein (arrow).)
Rabbit anti-Human HP Polyclonal Antibody | anti-HP antibody
HP Antibody (Center)
Gene Names
HP; BP; HPA1S; HP2ALPHA2
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HP, Antibody; HP Antibody (Center); Haptoglobin; Zonulin; Haptoglobin alpha chain; Haptoglobin beta chain; HP; anti-HP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-322 amino acids from the Central region of human HP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
296-322
Sequence Length
347
Applicable Applications for anti-HP antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(HP Antibody (Center) western blot analysis in MDA-MB453,CEM,NCI-H460,HepG2,K562 cell line lysates (35ug/lane).This demonstrates the HP antibody detected the HP protein (arrow).)
WB (Western Blot)
(HP Antibody (Center) western blot analysis in MDA-MB453,CEM,NCI-H460,HepG2,K562 cell line lysates (35ug/lane).This demonstrates the HP antibody detected the HP protein (arrow).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HP Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HP Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(HP Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HP Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(HP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(HP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of HP (arrow) using rabbit polyclonal HP Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene.)
WB (Western Blot)
(Western blot analysis of HP (arrow) using rabbit polyclonal HP Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene.)
Related Product Information for anti-HP antibody
This gene encodes a preproprotein, which is processed to
yield both alpha and beta chains, which subsequently combine as a
tetramer to produce haptoglobin. Haptoglobin functions to bind free
plasma hemoglobin, which allows degradative enzymes to gain access
to the hemoglobin, while at the same time preventing loss of iron
through the kidneys and protecting the kidneys from damage by
hemoglobin. Mutations in this gene and/or its regulatory regions
cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also
been linked to diabetic nephropathy, the incidence of coronary
artery disease in type 1 diabetes, Crohn's disease, inflammatory
disease behavior, primary sclerosing cholangitis, susceptibility to
idiopathic Parkinson's disease, and a reduced incidence of
Plasmodium falciparum malaria. A similar duplicated gene is located
next to this gene on chromosome 16. Multiple transcript variants
encoding different isoforms have been found for this gene.
yield both alpha and beta chains, which subsequently combine as a
tetramer to produce haptoglobin. Haptoglobin functions to bind free
plasma hemoglobin, which allows degradative enzymes to gain access
to the hemoglobin, while at the same time preventing loss of iron
through the kidneys and protecting the kidneys from damage by
hemoglobin. Mutations in this gene and/or its regulatory regions
cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also
been linked to diabetic nephropathy, the incidence of coronary
artery disease in type 1 diabetes, Crohn's disease, inflammatory
disease behavior, primary sclerosing cholangitis, susceptibility to
idiopathic Parkinson's disease, and a reduced incidence of
Plasmodium falciparum malaria. A similar duplicated gene is located
next to this gene on chromosome 16. Multiple transcript variants
encoding different isoforms have been found for this gene.
Product Categories/Family for anti-HP antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Savy, M., et al. PLoS ONE 5 (6), E11075 (2010) :
Kasvosve, I., et al. Adv Clin Chem 50, 23-46 (2010) :
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Savy, M., et al. PLoS ONE 5 (6), E11075 (2010) :
Kasvosve, I., et al. Adv Clin Chem 50, 23-46 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45205
NCBI Official Full Name
haptoglobin isoform 2 preproprotein
NCBI Official Synonym Full Names
haptoglobin
NCBI Official Symbol
HP
NCBI Official Synonym Symbols
BP; HPA1S; HP2ALPHA2
NCBI Protein Information
haptoglobin
UniProt Protein Name
Haptoglobin
UniProt Gene Name
HP
UniProt Entry Name
HPT_HUMAN
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Product Notes
The HP hp (Catalog #AAA288862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 296-322. The HP Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HP can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HP hp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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