Rabbit HPN / TMPRSS1 / Hepsin Polyclonal Antibody | anti-HPN antibody

Rabbit Polyclonal (IgG) to Human HPN / TMPRSS1 / Hepsin

Cat. Num. AAA51251

• Gene Names: HPN; TMPRSS1
• Reactivity: Human, Monkey, Bat, Bovine, Dog, Rabbit; Predicted Reactivity: Hamster, Horse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: HPN / TMPRSS1 / Hepsin, Antibody; Rabbit Polyclonal (IgG) to Human HPN / TMPRSS1 / Hepsin; Anti-HPN / TMPRSS1 / Hepsin Antibody IHC-plus; HPN; Hepsin; TMPRSS1; Serine protease hepsin; Human HPN; anti-HPN antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Bat, Bovine, Dog, Rabbit; Predicted Reactivity: Hamster, Horse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: TBS containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide
• Concentration: 0.4 mg/ml (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-HPN antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: Human hepsin amino acids 241-260 (GGYLPFRDPNSEENSNDIAL). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Bovine, Dog, Bat, Panda, Rabbit (100%); Opossum (95%); Hamster, Horse (90%); Mouse, Rat (85%); Xenopus (80%).
• Immunogen: HPN / TMPRSS1 / Hepsin antibody was raised against human hepsin amino acids 241-260 (GGYLPFRDPNSEENSNDIAL). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Bovine, Dog, Bat, Panda, Rabbit (100%); Opossum (95%); Hamster, Horse (90%); Mouse, Rat (85%); Xenopus (80%).
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C for up to 1 year.

IHC (Immunohistochemistry)

(Anti-HPN / Hepsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Product Categories/Family for anti-HPN antibody

Family: Protease; Subfamily: Serine S1

NCBI and Uniprot Product Information

• NCBI GI #: 4504481
• NCBI GeneID: 3249
• NCBI Accession #: NP_002142.1
• NCBI GenBank Nucleotide #: NM_002151.2
• UniProt Accession #: P05981; B2RDS4
• Molecular Weight: 45,011 Da
• NCBI Official Full Name: serine protease hepsin
• NCBI Official Synonym Full Names: hepsin
• NCBI Official Symbol: HPN
• NCBI Official Synonym Symbols: TMPRSS1
• NCBI Protein Information: serine protease hepsin; transmembrane protease serine 1
• UniProt Protein Name: Serine protease hepsin
• UniProt Gene Name: HPN
• UniProt Synonym Gene Names: TMPRSS1
• UniProt Entry Name: HEPS_HUMAN