WB (Western Blot)
(Detection of human HRS by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody (AAA211263 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse HRS Polyclonal Antibody | anti-HRS antibody
Rabbit anti-HRS Antibody, Affinity Purified
Gene Names
HGS; HRS
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
HRS, Antibody; Rabbit anti-HRS Antibody, Affinity Purified; protein pp110; hepatocyte growth factor-regulated tyrosine kinase substrate; HRS; human growth factor-regulated tyrosine kinase substrate; Hepatocyte growth factor-regulated tyrosine kinase substrate; anti-HRS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-HRS antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 727 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HRS by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody (AAA211263 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human HRS by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody (AAA211263 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of mouse HRS by western blot. Samples: Whole cell lysate (10 ug) from RenCa, CT26, 4T1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody (AAA211263 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse HRS by western blot. Samples: Whole cell lysate (10 ug) from RenCa, CT26, 4T1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody (AAA211263 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BeWo, AlphaTC1 Clone 9, A20, and 3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HRS antibody (AAA211263 lot 3) used for IP at 6 ug per reaction. HRS was also immunoprecipitated by a previous lot of this antibody (AAA211263 lot 2) and rabbit anti-HRS antibody For blotting immunoprecipitated HRS, AAA211263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BeWo, AlphaTC1 Clone 9, A20, and 3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HRS antibody (AAA211263 lot 3) used for IP at 6 ug per reaction. HRS was also immunoprecipitated by a previous lot of this antibody (AAA211263 lot 2) and rabbit anti-HRS antibody For blotting immunoprecipitated HRS, AAA211263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-HRS antibody
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) is an endosomal protein that participates in the trafficking and sorting of proteins to degradative or recycling pathways. HRS can bind ubiquitin directly and plays a key role in sorting ubiquitinated proteins to lysosomes. HRS has also been demonstrated to regulate protein sorting independent of its ubiquitin-binding motif.
Product Categories/Family for anti-HRS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86,192 Da
NCBI Official Full Name
hepatocyte growth factor-regulated tyrosine kinase substrate
NCBI Official Synonym Full Names
hepatocyte growth factor-regulated tyrosine kinase substrate
NCBI Official Symbol
HGS
NCBI Official Synonym Symbols
HRS
NCBI Protein Information
hepatocyte growth factor-regulated tyrosine kinase substrate; protein pp110; human growth factor-regulated tyrosine kinase substrate
UniProt Protein Name
Hepatocyte growth factor-regulated tyrosine kinase substrate
UniProt Gene Name
HGS
UniProt Synonym Gene Names
HRS
UniProt Entry Name
HGS_HUMAN
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Product Notes
The HRS hgs (Catalog #AAA211263) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HRS Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HRS can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HRS hgs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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