WB (Western Blot)
(Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody AAA212135 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HSF1 was also immunoprecipitated by rabbit anti-HSF1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
Rabbit anti-Human HSF1 Polyclonal Antibody | anti-HSF1 antibody
Rabbit anti-HSF1 Antibody, Affinity Purified
Gene Names
HSF1; HSTF1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
HSF1, Antibody; Rabbit anti-HSF1 Antibody, Affinity Purified; HSTF1; heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSTF 1; Heat shock factor protein 1; heat shock transcription factor 1; anti-HSF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-HSF1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 479 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody AAA212135 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HSF1 was also immunoprecipitated by rabbit anti-HSF1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody AAA212135 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HSF1 was also immunoprecipitated by rabbit anti-HSF1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
IHC (Immunohiostchemistry)
(Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSF1 Cat. No. Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. No. AAA212135 Lot1 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSF1 Cat. No. Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. No. AAA212135 Lot1 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-HSF1 Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (center) and Cat. No. AAA212135 Lot1 used at a dilution of 1:200 (1ug/ml) (right). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-HSF1 Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (center) and Cat. No. AAA212135 Lot1 used at a dilution of 1:200 (1ug/ml) (right). Detection: DAB)
Related Product Information for anti-HSF1 antibody
Heat shock factor protein 1 (HSF1) is a DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q00613].
Product Categories/Family for anti-HSF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,260 Da
NCBI Official Full Name
heat shock factor protein 1
NCBI Official Synonym Full Names
heat shock transcription factor 1
NCBI Official Symbol
HSF1
NCBI Official Synonym Symbols
HSTF1
NCBI Protein Information
heat shock factor protein 1; HSF 1; HSTF 1
UniProt Protein Name
Heat shock factor protein 1
UniProt Gene Name
HSF1
UniProt Synonym Gene Names
HSTF1; HSF 1; HSTF 1
UniProt Entry Name
HSF1_HUMAN
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Product Notes
The HSF1 hsf1 (Catalog #AAA212135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HSF1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSF1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HSF1 hsf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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