ICC (Immunocytochemistry)
(Anti-HSP60 antibody, AAA45054, ICCICC: SMMC Cell)
Rabbit HSP60 Polyclonal Antibody | anti-HSPD1 antibody
Anti-HSP60 antibody
Gene Names
Hspd1; Hsp60; Hspd1-30p
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
HSP60, Antibody; Anti-HSP60 antibody; 60 kDa heat shock protein, mitochondrial; heat shock 60kDa protein 1(chaperonin); 60 kDa chaperonin antibody; 60 kDa heat shock protein mitochondrial antibody; 60 kDa heat shock protein, mitochondrial antibody; CH60_HUMAN antibody; Chaperonin 60 antibody; Chaperonin antibody; Chaperonin, 60-KD antibody; CPN 60 antibody; CPN60 antibody; fa04a05 antibody; GROEL antibody; GroEL Homolog antibody; Heat shock 60kD protein 1 chaperonin antibody; Heat shock 60kDa protein 1 antibody; Heat shock protein 1(chaperonin) antibody; Heat Shock Protein 60 antibody; Heat shock protein 65 antibody; HLD4 antibody; Hsp 60 antibody; HSP 65 antibody; HSP-60 antibody; HSP60 antibody; HSP65 antibody; HSPD 1 antibody; HSPD1 antibody; HuCHA60 antibody; Mitochondrial matrix protein P1 antibody; P60 lymphocyte protein antibody; Short heat shock protein 60 Hsp60s1 antibody; Spastic paraplegia 13 antibody; SPG 13 antibody; SPG13 antibody; anti-HSPD1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
573
Applicable Applications for anti-HSPD1 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60 (503-516aa YDAMLGDFVNMVEK), different from the related human sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti-HSP60 antibody, AAA45054, ICCICC: SMMC Cell)
ICC (Immunocytochemistry)
(Anti-HSP60 antibody, AAA45054, ICCICC: SMMC Cell)
IHC (Immunohiostchemistry)
(Anti-HSP60 antibody, AAA45054, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti-HSP60 antibody, AAA45054, IHC(P)IHC(P): Rat Intestine Tissue)
WB (Western Blot)
(Anti-HSP60 antibody, AAA45054, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: CEM Cell LysateLane 4: HELA Cell LysateLane 5: SMMC Cell LysateLane 6: COLO320 Cell Lysate)
WB (Western Blot)
(Anti-HSP60 antibody, AAA45054, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: CEM Cell LysateLane 4: HELA Cell LysateLane 5: SMMC Cell LysateLane 6: COLO320 Cell Lysate)
Related Product Information for anti-HSPD1 antibody
Description: Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
Background: HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,955 Da
NCBI Official Full Name
60 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein 1 (chaperonin)
NCBI Official Symbol
Hspd1
NCBI Official Synonym Symbols
Hsp60; Hspd1-30p
NCBI Protein Information
60 kDa heat shock protein, mitochondrial; CPN60; HSP-60; HSP-65; chaperonin 60; 60 kDa chaperonin; heat shock 60kDa protein 1; heat shock protein 60 (liver); mitochondrial matrix protein P1; heat shock 60kD protein 1 (chaperonin)
UniProt Protein Name
60 kDa heat shock protein, mitochondrial
UniProt Gene Name
Hspd1
UniProt Synonym Gene Names
Hsp60; CPN60; HSP-60; Hsp60
UniProt Entry Name
CH60_RAT
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Product Notes
The HSPD1 hspd1 (Catalog #AAA45054) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HSP60 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSP60 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HSPD1 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP60, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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