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IF (Immunofluorescence)
(AAA61783 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
Goat HSP60/HSPD1 Polyclonal Antibody | anti-HSPD1 antibody
Goat anti-HSP60/HSPD1 (aa333-344) Antibody
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Tested: Human, Rat, PigExpected from sequence similarity: Human, Dog, Pig, Cow
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
HSP60/HSPD1, Antibody; Goat anti-HSP60/HSPD1 (aa333-344) Antibody; HSPD1; heat shock 60kDa protein 1 (chaperonin); CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13; 60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; chaperonin 60; heat shock protein 65; mitochondrial matrix prote; HSPD1 antibody; heat shock 60kDa protein 1 (chaperonin) antibody; CPN60 antibody; GROEL antibody; HLD4 antibody; HSP-60 antibody; HSP60 antibody; HSP65 antibody; HuCHA60 antibody; SPG13 antibody; 60 kDa chaperonin antibody; 60 kDa heat shock protein; mitochondrial antibody; P60 lymphocyte protein antibody; chaperonin 60 antibody; heat shock protein 65 antibody; mitochondrial matrix prote antibody; HSP60 / HSPD1 (aa333-344); anti-HSPD1 antibody
Host
Goat
Reactivity
Tested: Human, Rat, Pig
Expected from sequence similarity: Human, Dog, Pig, Cow
Expected from sequence similarity: Human, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
C-NLEDVQPHDLGK
Sequence Length
573
Applicable Applications for anti-HSPD1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-NLEDVQPHDLGK, from the internal region of the protein sequence according to NP_002147.2.
Note
Reported variants represent identical protein: NP_955472.1, NP_002147.2.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1052
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IF (Immunofluorescence)
(AAA61783 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
IF (Immunofluorescence)
(AAA61783 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
IHC (Immunohistochemisry)
(AAA61783 (5 μg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemisry)
(AAA61783 (5 μg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
WB (Western Blot)
(AAA61783 (0.1ug/ml) staining of Rat Kidney lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
(AAA61783 (0.1ug/ml) staining of Rat Kidney lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
(AAA61783 (0.03 μg/ml) staining of Human Heart (A), Kidney (B) and Liver (C) lysate and in Pig Heart (D), Kidney(E) and Liver (F) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
(AAA61783 (0.03 μg/ml) staining of Human Heart (A), Kidney (B) and Liver (C) lysate and in Pig Heart (D), Kidney(E) and Liver (F) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
60 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein family D (Hsp60) member 1
NCBI Official Symbol
HSPD1
NCBI Official Synonym Symbols
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
NCBI Protein Information
60 kDa heat shock protein, mitochondrial
UniProt Protein Name
60 kDa heat shock protein, mitochondrial
UniProt Gene Name
HSPD1
UniProt Synonym Gene Names
HSP60; CPN60; HSP-60; Hsp60
UniProt Entry Name
CH60_HUMAN
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Product Notes
The HSPD1 hspd1 (Catalog #AAA61783) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HSP60/HSPD1 (aa333-344) Antibody reacts with Tested: Human, Rat, Pig Expected from sequence similarity: Human, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's HSP60/HSPD1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the HSPD1 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: C-NLEDVQPH DLGK. It is sometimes possible for the material contained within the vial of "HSP60/HSPD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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