WB (Western Blot)
(Anti-Hsp90 Antibody - Western Blot. Western blot of Affinity Purified anti-Hsp90 antibody shows detection of a band at ~90 kD corresponding to Hsp90 in various lysate preparations (arrowhead). Cos7 transfected with mutant K294R Hsp90 (left two lanes), Cos7 transfected with wild type Hsp90 (middle two lanes) and empty vector Cos7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated. Transfected cells express either mutant or wt Hsp90 coupled to FlagTM tag. Anti-FlagTM immunoprecipitation was performed prior to western blotting with anti-Hsp90. Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD.)
Rabbit HSP90AA1 / Hsp90 Polyclonal Antibody | anti-HSP90AA1 antibody
Rabbit Polyclonal to Human HSP90AA1 / Hsp90
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Reactivity
Chicken, Drosophila, Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
HSP90AA1 / Hsp90, Antibody; Rabbit Polyclonal to Human HSP90AA1 / Hsp90; Anti-HSP90AA1 / Hsp90 Antibody (aa289-300) IHC-plus; HSP90AA1; EL52; HSP86; Hsp89; HSP89A; HSPC1; Heat shock 86 kDa; Hsp90; HSP90A; HSP90N; HSPCA; HSPCAL4; HSPN; LAP2; HSP 86; HSPCAL1; Human HSP90AA1; anti-HSP90AA1 antibody
Host
Rabbit
Reactivity
Chicken, Drosophila, Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Specificity
Amino acids 289-300 of human Hsp90 protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
732
Applicable Applications for anti-HSP90AA1 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human HSP90AA1 / Hsp90.
Usage Summary
Immunohistochemistry: MBS240566 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS240566 was determined to be 2.5 ug/ml.
Summary
Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species. Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1 alpha). Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG). The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out). One possible mechanism may be differences in post-translational modification of tumor Hsp90. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo, and interacts with TOM34, AHSA1, HDAC6 and SMYD3 .
Immunogen Type
Synthetic peptide
Immunogen
HSP90AA1 / Hsp90 antibody was raised against synthetic peptide from human HSP90AA1 / Hsp90.
Antigen Modification
aa289-300
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
WB (Western Blot)
(Anti-Hsp90 Antibody - Western Blot. Western blot of Affinity Purified anti-Hsp90 antibody shows detection of a band at ~90 kD corresponding to Hsp90 in various lysate preparations (arrowhead). Cos7 transfected with mutant K294R Hsp90 (left two lanes), Cos7 transfected with wild type Hsp90 (middle two lanes) and empty vector Cos7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated. Transfected cells express either mutant or wt Hsp90 coupled to FlagTM tag. Anti-FlagTM immunoprecipitation was performed prior to western blotting with anti-Hsp90. Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD.)
WB (Western Blot)
(Anti-Hsp90 Antibody - Western Blot. Western blot of Affinity Purified anti-Hsp90 antibody shows detection of a band at ~90 kD corresponding to Hsp90 in various lysate preparations (arrowhead). Cos7 transfected with mutant K294R Hsp90 (left two lanes), Cos7 transfected with wild type Hsp90 (middle two lanes) and empty vector Cos7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated. Transfected cells express either mutant or wt Hsp90 coupled to FlagTM tag. Anti-FlagTM immunoprecipitation was performed prior to western blotting with anti-Hsp90. Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD.)
IHC (Immunohistochemistry)
(Anti-HSP90AA1 / Hsp90 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HSP90AA1 / Hsp90 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
Related Product Information for anti-HSP90AA1 antibody
HSP90AA1 is a member of the group of proteins produced by cells to deal with stress such as elevated temperature. It is known to chaperone a key subset of signaling proteins and is necessary for malignant transformation. HSP90 is subject to an array of post translational modifications that affect its function including acetylation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,161 Da
NCBI Official Full Name
heat shock protein HSP 90-alpha isoform 2
NCBI Official Synonym Full Names
heat shock protein 90kDa alpha (cytosolic), class A member 1
NCBI Official Symbol
HSP90AA1
NCBI Official Synonym Symbols
EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
NCBI Protein Information
heat shock protein HSP 90-alpha; HSP 86; heat shock 86 kDa; LPS-associated protein 2; heat shock 90kD protein 1, alpha; heat shock 90kDa protein 1, alpha; renal carcinoma antigen NY-REN-38; heat shock 90kD protein, alpha-like 4; epididymis luminal secretory protein 52; heat shock 90kD protein 1, alpha-like 4; lipopolysaccharide-associated protein 2
UniProt Protein Name
Heat shock protein HSP 90-alpha
UniProt Gene Name
HSP90AA1
UniProt Synonym Gene Names
HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2
UniProt Entry Name
HS90A_HUMAN
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Product Notes
The HSP90AA1 hsp90aa1 (Catalog #AAA50744) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human HSP90AA1 / Hsp90 reacts with Chicken, Drosophila, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSP90AA1 / Hsp90 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the HSP90AA1 hsp90aa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP90AA1 / Hsp90, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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