Rabbit anti-Human, mouse HSPA5 Polyclonal Antibody | anti-HSPA5 antibody

HSPA5 Antibody

Cat. Num. AAA28738

• Gene Names: HSPA5; BIP; MIF2; GRP78; HEL-S-89n
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HSPA5; Polyclonal Antibody; HSPA5 Antibody; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; GRP78; anti-HSPA5 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Length: 654

• Applicable Applications for anti-HSPA5 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Recombinant Protein
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM (Flow Cytometry)

(HSPA5 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of HSPA5 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of anti-HSPA5 Pab in HL60 cell line lysates (35ug/lane).HSPA5(arrow) was detected using the purified Pab.)

WB (Western Blot)

(The anti-HSPA5 Pab is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.)

Related Product Information for anti-HSPA5 antibody

In cooperation with other chaperones, HSP70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. The HSP70s in mitochondria and the endoplasmic reticulum play an additional role by providing a driving force for protein translocation. They are involved in signal transduction pathways in cooperation with HSP90. They participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Milner, C.M., et al., Immunogenetics 32(4):242-251 (1990). Sargent, C.A., et al., Proc. Natl. Acad. Sci. U.S.A. 86(6):1968-1972 (1989). Drabent, B., et al., Nucleic Acids Res. 14(22):8933-8948 (1986). Hunt, C., et al., Proc. Natl. Acad. Sci. U.S.A. 82(19):6455-6459 (1985).

NCBI and Uniprot Product Information

• NCBI GI #: 16507237
• NCBI GeneID: 3309
• NCBI Accession #: NP_005338.1
• NCBI GenBank Nucleotide #: NM_005347.4
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 72333
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; HEL-S-89n
• NCBI Protein Information: 78 kDa glucose-regulated protein
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78; GRP-78; BiP
• UniProt Entry Name: GRP78_HUMAN