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IHC (Immunohistochemistry)
(IHC diluted at 1:150 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Rabbit human Protein AMBP Polyclonal Antibody | anti-AMBP antibody
Rabbit anti-human human Protein AMBP polyclonal Antibody
Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
human Protein AMBP, Antibody; Rabbit anti-human human Protein AMBP polyclonal Antibody; Alternative name(s): Alpha-1 microglycoprotein Complex-forming glycoprotein heterogeneous in charge Bikunin EDC1 HI-30 Uronic-acid-rich protein AMBP HCP; ITIL; anti-AMBP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Sequence Length
352
Applicable Applications for anti-AMBP antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Protein AMBP protein (20-203AA)
Conjugation
Non-conjugated
Immunogen Species
Homo sapiens (Human)
Research Area
Cardiovascular
Target Names
AMBP
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21598 / sc-21597
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid repeated freeze
IHC (Immunohistochemistry)
(IHC diluted at 1:150 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC diluted at 1:150 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: HepG2 whole cell lysate, U251 whole cell lysateAll lanes: AMBP antibody at 1:1000Secondary Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39 kDaObserved band size: 39 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: HepG2 whole cell lysate, U251 whole cell lysateAll lanes: AMBP antibody at 1:1000Secondary Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39 kDaObserved band size: 39 kDa)
WB (Western Blot)
(Western blotAll lanes: AMBP antibody at 1.5ug/ml+Mouse liver tissueGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 39 kDaObserved band size: 39 kDa)
WB (Western Blot)
(Western blotAll lanes: AMBP antibody at 1.5ug/ml+Mouse liver tissueGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 39 kDaObserved band size: 39 kDa)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA229841 at dilution of 1:20)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA229841 at dilution of 1:20)
Product Categories/Family for anti-AMBP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein AMBP preproprotein
NCBI Official Synonym Full Names
alpha-1-microglobulin/bikunin precursor
NCBI Official Symbol
AMBP
NCBI Official Synonym Symbols
A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
NCBI Protein Information
protein AMBP
UniProt Protein Name
Protein AMBP
UniProt Gene Name
AMBP
UniProt Synonym Gene Names
HCP; ITIL; Protein HC; ITI-LC
UniProt Entry Name
AMBP_HUMAN
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Product Notes
The AMBP ambp (Catalog #AAA229841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human human Protein AMBP polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's human Protein AMBP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the AMBP ambp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "human Protein AMBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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