WB (Western Blot)
(Detection of human HURP/DLG7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HURP/DLG7 antibody AAA211180 (lot AAA211180-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human HURP/DLG7 Polyclonal Antibody | anti-HURP/DLG7 antibody
Rabbit anti-HURP/DLG7 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
HURP/DLG7, Antibody; Rabbit anti-HURP/DLG7 Antibody, Affinity Purified; hepatoma up-regulated protein; HURP; DLG7; disks large-associated protein DLG7; discs, large homolog 7; discs large homolog associated protein 5; DAP-5; Discs large homolog 7; disks large-associated protein 5; Disks large-associated protein 5; DLG associated protein 5; anti-HURP/DLG7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-HURP/DLG7 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HURP/DLG7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HURP/DLG7 antibody AAA211180 (lot AAA211180-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human HURP/DLG7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HURP/DLG7 antibody AAA211180 (lot AAA211180-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human HURP/DLG7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HURP/DLG7 antibody AAA211180 (lot AAA211180-2) used for IP at 6 ug per reaction. HURP/DLG7 was also immunoprecipitated by a previous lot of this antibody (AAA211180-1) and rabbit anti-HURP/DLG7 antibody For blotting immunoprecipitated HURP/DLG7, AAA211180 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human HURP/DLG7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HURP/DLG7 antibody AAA211180 (lot AAA211180-2) used for IP at 6 ug per reaction. HURP/DLG7 was also immunoprecipitated by a previous lot of this antibody (AAA211180-1) and rabbit anti-HURP/DLG7 antibody For blotting immunoprecipitated HURP/DLG7, AAA211180 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human HURP/DLG7 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HURP/DLG7 (Cat. No. AAA211180 Lot2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human HURP/DLG7 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HURP/DLG7 (Cat. No. AAA211180 Lot2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-HURP/DLG7 antibody
HURP/DLG7 (Hepatoma Up-Regulated Protein/Disks Large Homolog 7) is a member of the MAGUK (membrane-associated guanylate kinase) family of scaffolding proteins. There are seven DLG homologs in mammals that play important roles in regulating proliferation, epithelial cell polarity, cell adhesion, and synapse formation. HURP/DLG7 has been identified as a microtubule-associated protein (MAP) that colocalizes with the mitotic spindle and functions as a mitotic regulator. HURP/DLG7 is required for microtubule attachment and efficient capture of kinetochores, as well as timely chromosome congression. HURP/DLG7 is up-regulated in human cancer cells and may contribute to tumorigenesis.
Product Categories/Family for anti-HURP/DLG7 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HURP/DLG7 dlgap5 (Catalog #AAA211180) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HURP/DLG7 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HURP/DLG7 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HURP/DLG7 dlgap5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HURP/DLG7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.