Application Data
(WB: Tissue lysate from hypoxic mouse brain (lane 2) was resolved onto 10% SDS-PAGE and transferred onto NC Membrane, then immune-probed by Anti-HYOU1 antibody, at 1:500. An immunoreactive protein is observed around ~150kDa)
Rabbit HYOU1 Polyclonal Antibody | anti-HYOU1 antibody
Rabbit anti HYOU1 Polyclonal Antibody
Gene Names
HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, ELISA
Purity
Purified by Epitope Affinity Purification
Synonyms
HYOU1, Antibody; Rabbit anti HYOU1 Polyclonal Antibody; Hypoxia up-regulated 1; anti-HYOU1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes ~150 kDa of HYOU1 Protein. It cross-reacts with human, mouse and rat. The other species are not tested.
Purity/Purification
Purified by Epitope Affinity Purification
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Sequence Length
657
Applicable Applications for anti-HYOU1 antibody
WB (Western Blot), IP (Immunoprecipitation), ELISA
Preparation
A synthetic peptide next to the C-terminus of HYOU1 protein. This sequence is identical to rat, mouse, human and dog, bovine and horse origins.
Positive Control
Hypoxia mouse brain
Cell Location
Cytoplasmic/ER
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70°C. The antibodies can be stored at 2°C - 8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Application Data
(WB: Tissue lysate from hypoxic mouse brain (lane 2) was resolved onto 10% SDS-PAGE and transferred onto NC Membrane, then immune-probed by Anti-HYOU1 antibody, at 1:500. An immunoreactive protein is observed around ~150kDa)
Application Data
(WB: Tissue lysate from hypoxic mouse brain (lane 2) was resolved onto 10% SDS-PAGE and transferred onto NC Membrane, then immune-probed by Anti-HYOU1 antibody, at 1:500. An immunoreactive protein is observed around ~150kDa)
Related Product Information for anti-HYOU1 antibody
The Hypoxia up-regulated 1 (HYOU1) protein belongs to the heat shock protein 70 family. It plays an important role in protein folding and secretion in the endoplasmic reticulum (ER). This protein is involved in stress-dependent induction, resulting in the accumulation of this protein in the ER under hypoxic conditions. It also has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness.
References
Bando, Y., et al. 150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells. JOURNAL Am. J. Physiol., Cell Physiol. 278 (6), C1172-C1182 (2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~150 kDa
NCBI Official Full Name
HYOU1 protein, partial
NCBI Official Synonym Full Names
hypoxia up-regulated 1
NCBI Official Symbol
HYOU1
NCBI Official Synonym Symbols
Grp170; HSP12A; ORP150; GRP-170; ORP-150
NCBI Protein Information
hypoxia up-regulated protein 1
UniProt Protein Name
Hypoxia up-regulated protein 1
UniProt Gene Name
HYOU1
UniProt Synonym Gene Names
GRP170; ORP150; ORP-150; GRP-170
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Product Notes
The HYOU1 hyou1 (Catalog #AAA71326) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti HYOU1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HYOU1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), ELISA. Researchers should empirically determine the suitability of the HYOU1 hyou1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HYOU1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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