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IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA229166 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human Hypoxia-inducible factor 1-alpha Polyclonal Antibody | anti-HIF1A antibody
Rabbit anti-human Hypoxia-inducible factor 1-alpha polyclonal Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA
Purity
>95%, Protein G Purified
Synonyms
Hypoxia-inducible factor 1-alpha, Antibody; Rabbit anti-human Hypoxia-inducible factor 1-alpha polyclonal Antibody; Hypoxia-inducible factor 1-alpha (HIF-1-alpha) (HIF1-alpha) (ARNT-interacting rotein) (Basic-helix-loop-helix-PAS protein MOP1) (Class E basic helix-loophelix protein 78) (bHLHe78) (Member of PAS protein 1) (PAS domain-containing protein 8), HIF1A, BHLHE78 MOP1 PASD8; anti-HIF1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G Purified
Form/Format
Liquid
Applicable Applications for anti-HIF1A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant Human Hypoxia-inducible factor 1-alpha protein (579-826AA)
Conjugate
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Species
Homo sapiens (Human)
Research Area
Cardiovascular
Target Names
HIF1A
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA229166 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA229166 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of human colon cancer using AAA229166 at dilution of 1:100)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of human colon cancer using AAA229166 at dilution of 1:100)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of human bladder cancer using AAA229166 at dilution of 1:100)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of human bladder cancer using AAA229166 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA229166 at dilution 1:50)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA229166 at dilution 1:50)
WB (Western Blot)
(All lanes: Hypoxia-inducible factor 1-alpha antibody at 2ug/mlLane 1: HGC27 whole cell lysateLane 2: A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 93,83,96 kDaObserved band size: 93 kDa)
WB (Western Blot)
(All lanes: Hypoxia-inducible factor 1-alpha antibody at 2ug/mlLane 1: HGC27 whole cell lysateLane 2: A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 93,83,96 kDaObserved band size: 93 kDa)
Related Product Information for anti-HIF1A antibody
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Product Categories/Family for anti-HIF1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 3
NCBI Official Synonym Full Names
hypoxia inducible factor 1 alpha subunit
NCBI Official Symbol
HIF1A
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
NCBI Protein Information
hypoxia-inducible factor 1-alpha
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
HIF1A
UniProt Synonym Gene Names
BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
UniProt Entry Name
HIF1A_HUMAN
Similar Products
Product Notes
The HIF1A hif1a (Catalog #AAA229166) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Hypoxia-inducible factor 1-alpha polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hypoxia-inducible factor 1-alpha can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the HIF1A hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hypoxia-inducible factor 1-alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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