Hypoxia Inducible Factor 1 Alpha (HIF1a) Recombinant Protein | HIF1a recombinant protein

Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)

Cat. Num. AAA134348

• Gene Names: HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
• Applications: Immunoprecipitation, ELISA, Western Blot, SDS-Page
• Purity: > 95%
• Synonyms: Hypoxia Inducible Factor 1 Alpha (HIF1a); N/A; Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a); HIF1a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-RSFDQL SPLESSSASP ESASPQSTVT VFQQTQIQEP TANATTTTAT TDELKTVTKD RMEDIKILIA SPSPTHIHKE TTSATSSPYR DTQSRTASPN RAGKGVIEQT EKSHPRSPNV LSVALSQRTT VPEEELNPKI LALQNAQRKR KMEHDGSLFQ AVGIGTLLQQ PDDHAATTSL SWKRVKGCKS SEQNGMEQKT IILIPSDLAC RLLGQSMDES GLPQLTSYDC EVNAPIQGSR NLLQGEELLR ALDQVN
• Sequence Length: 850

• Applicable Applications for HIF1a recombinant protein: IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Arg575~Asn826 (Accession # Q16665) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Cytoplasm. Nucleus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Application Data

SDS-PAGE

Sequence

NCBI and Uniprot Product Information

• NCBI GI #: 340545531
• NCBI GeneID: 3091
• NCBI Accession #: NP_001230013.1
• NCBI GenBank Nucleotide #: NM_001243084.1
• UniProt Accession #: Q16665; Q53XP6; Q96PT9; Q9UPB1; C0LZJ3
• Molecular Weight: 59.4kDa
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 3
• NCBI Official Synonym Full Names: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• NCBI Official Symbol: HIF1A
• NCBI Official Synonym Symbols: HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
• NCBI Protein Information: hypoxia-inducible factor 1-alpha; HIF-1-alpha; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; hypoxia-inducible factor1alpha; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
• UniProt Entry Name: HIF1A_HUMAN