WB (Western Blot)
(Detection of human I kappa B-beta by western blot. Samples: Whole cell lysate from HeLa (50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-I kappa B-beta antibody AAA211647 (lot AAA211647-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 3 minutes.)
Rabbit anti-Human I kappa B-beta Polyclonal Antibody | anti-IkappaB-beta antibody
Rabbit anti-I kappa B-beta Antibody, Affinity Purified
Gene Names
NFKBIB; IKBB; TRIP9
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
I kappa B-beta, Antibody; Rabbit anti-I kappa B-beta Antibody, Affinity Purified; ikB-beta; TRIP-9; TRIP9; TR-interacting protein 9; thyroid receptor-interacting protein 9; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta; NF-kappa-B inhibitor beta; ikB-B; IKBB; I-kappa-B-beta; ikappaBbeta; NF-kappa-BIB; NFKB inhibitor beta; anti-IkappaB-beta antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-IkappaB-beta antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 306 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human I kappa B-beta by western blot. Samples: Whole cell lysate from HeLa (50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-I kappa B-beta antibody AAA211647 (lot AAA211647-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human I kappa B-beta by western blot. Samples: Whole cell lysate from HeLa (50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-I kappa B-beta antibody AAA211647 (lot AAA211647-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human I kappa B-beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-I kappa B-beta antibody AAA211647 (lot AAA211647-1) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated I kappa B-beta, AAA211647 was used at 0.4 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human I kappa B-beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-I kappa B-beta antibody AAA211647 (lot AAA211647-1) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated I kappa B-beta, AAA211647 was used at 0.4 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
Related Product Information for anti-IkappaB-beta antibody
Activity of the transcription factor NF-kappa B is regulated by inhibitor proteins that interact with NF-kappa B and inhibit their DNA binding activity. These inhibitor proteins are members of the I kappa B family of proteins and include I kappa B-beta, I kappa B-delta/NF-kappa B2/p100, I kappa B-gamma/NF-kappa Bp105, I kappa B-epsilon, I kappa B-zeta, and Bcl3. Association with I kappa B proteins sequesters NF-kappa B in the cytoplasm to regulate its activity. The Phosphorylation of I kappa B by the I kappa B kinase complex (IKK) results in the ubiquitination and degradation of I kappa B and the release and translocation of NF-kappa B to the nucleus.
Product Categories/Family for anti-IkappaB-beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,530 Da
NCBI Official Full Name
NF-kappa-B inhibitor beta isoform 1
NCBI Official Synonym Full Names
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
NCBI Official Symbol
NFKBIB
NCBI Official Synonym Symbols
IKBB; TRIP9
NCBI Protein Information
NF-kappa-B inhibitor beta; ikB-B; TRIP-9; ikB-beta; ikappaBbeta; NF-kappa-BIB; I-kappa-B-beta; TR-interacting protein 9; thyroid receptor-interacting protein 9
UniProt Protein Name
NF-kappa-B inhibitor beta
UniProt Gene Name
NFKBIB
UniProt Synonym Gene Names
IKBB; TRIP9; NF-kappa-BIB; IkB-B; IkB-beta; IkappaBbeta; TR-interacting protein 9; TRIP-9
UniProt Entry Name
IKBB_HUMAN
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Product Notes
The IkappaB-beta nfkbib (Catalog #AAA211647) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-I kappa B-beta Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's I kappa B-beta can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IkappaB-beta nfkbib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "I kappa B-beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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