WB (Western Blot)
(Western blot analysis of chicken pAb to IBA1, AAA76710, dilution 1:1,000 in green: [1] protein standard (red) and [2] rat spleen crude homogenate. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates.)
Chicken IBA1 Polyclonal Antibody | anti-IBA1 antibody
IBA1
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Synonyms
IBA1, Antibody; IBA1; anti-IBA1 antibody
Host
Chicken
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Chicken IgY
Form/Format
Liquid. Supplied as an aliquot of IgYpreparation plus 5mM NaN3
Applicable Applications for anti-IBA1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Peptide identical to part of the C-terminal of human IBA1 coupled to KLH
Preparation and Storage
The antibody can be stored at 2 degree - 8 degree C for 12 months without detectable loss of activity. Avoidrepeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of chicken pAb to IBA1, AAA76710, dilution 1:1,000 in green: [1] protein standard (red) and [2] rat spleen crude homogenate. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates.)
WB (Western Blot)
(Western blot analysis of chicken pAb to IBA1, AAA76710, dilution 1:1,000 in green: [1] protein standard (red) and [2] rat spleen crude homogenate. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates.)
Application Data
(Rat spinal cord section, stained with AAA76710, dilution 1:1,000, in red. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Nuclear DNA is shown with DAPI stain in blue.)
Application Data
(Rat spinal cord section, stained with AAA76710, dilution 1:1,000, in red. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Nuclear DNA is shown with DAPI stain in blue.)
Related Product Information for anti-IBA1 antibody
IBA1 is an acronyn for "ionized Calcium binding adapter molecule 1", and the protein is also known as AIF1 for "allograft inflammatory factor 1". AIF1 was originally identified, cloned and sequenced as a protein heavily upregulated in an animal model of graft rejection. Suitable IBA1 antibodies are widely used to identify microglial cells in sections and tissues. In tissue samples from which they have not been washed out by perfusion, lymphocytes within blood vessels are also IBA1 positive. Microglia are the immunocompetent cells of the CNS and are extremely important in responses to injury and disease. Microglial are small but very active cells which constantly send processes probing their neighborhood and which alter morphology and are induced to divide following a variety of CNS compromises. Many important and highly cited papers have made use of IBA1 antibodies as markers of microglia.
This antibody was made against the C-terminal peptide of human IBA1 coupled to keyhole limpet hemocyanin. It works well on western blots, on cells cultures and sectioned material.
This antibody was made against the C-terminal peptide of human IBA1 coupled to keyhole limpet hemocyanin. It works well on western blots, on cells cultures and sectioned material.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
147
NCBI Official Full Name
Allograft inflammatory factor 1
NCBI Official Synonym Full Names
allograft inflammatory factor 1
NCBI Official Symbol
AIF1
NCBI Official Synonym Symbols
IBA1; IRT1; AIF-1; IRT-1
NCBI Protein Information
allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1
UniProt Protein Name
Allograft inflammatory factor 1
UniProt Gene Name
AIF1
UniProt Synonym Gene Names
G1; IBA1; AIF-1
UniProt Entry Name
AIF1_HUMAN
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Product Notes
The IBA1 aif1 (Catalog #AAA76710) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The IBA1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IBA1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the IBA1 aif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IBA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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