WB (Western Blot)
(AAA61634 (0.5ug/ml) staining of S. cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands)
Goat IDH1 Polyclonal Antibody | anti-IDH1 antibody
Goat anti-IDH1 (yeast) Antibody
Reactivity
Tested: S. cerevisiae S288c; Expected from sequence similarity: Saccharomyces cerevisiae
Applications
Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
IDH1, Antibody; Goat anti-IDH1 (yeast) Antibody; IDH1; Idh1p; YNL037C; Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; IDH1 antibody; Idh1p antibody; YNL037C antibody; Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle antibody; IDH1 (yeast); anti-IDH1 antibody
Host
Goat
Reactivity
Tested: S. cerevisiae S288c; Expected from sequence similarity: Saccharomyces cerevisiae
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EPGSRHVGLDIKGQN
Sequence Length
360
Applicable Applications for anti-IDH1 antibody
WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-EPGSRHVGLDIKGQN, from the internal region of the protein sequence according to NP_014361.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
WB (Western Blot)
(AAA61634 (0.5ug/ml) staining of S. cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands)
WB (Western Blot)
(AAA61634 (0.5ug/ml) staining of S. cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
isocitrate dehydrogenase (NAD(+)) IDH1
NCBI Official Symbol
IDH1
NCBI Protein Information
isocitrate dehydrogenase (NAD(+)) IDH1
UniProt Protein Name
Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial
UniProt Gene Name
IDH1
UniProt Entry Name
IDH1_YEAST
Similar Products
Product Notes
The IDH1 idh1 (Catalog #AAA61634) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IDH1 (yeast) Antibody reacts with Tested: S. cerevisiae S288c; Expected from sequence similarity: Saccharomyces cerevisiae and may cross-react with other species as described in the data sheet. AAA Biotech's IDH1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the IDH1 idh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EPGSRHVGLD IKGQN. It is sometimes possible for the material contained within the vial of "IDH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.