Mouse IDH1 (Isocitrate Dehydrogenase) Monoclonal Antibody | anti-IDH1 antibody

IDH1 (Isocitrate Dehydrogenase) Mouse Monoclonal Antibody

Cat. Num. AAA62882

• Gene Names: IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• Reactivity: Human.; Others not known
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: IDH1 (Isocitrate Dehydrogenase), Antibody; IDH1 (Isocitrate Dehydrogenase) Mouse Monoclonal Antibody; Cytosolic NADP-isocitrate dehydrogenase; Epididymis luminal protein 216; Epididymis secretory protein Li 26; HEL-216; HEL-S-26; ICDH; IDCD; IDH1; IDP; IDPC; Isocitrate dehydrogenase [NADP] cytoplasmic; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP dependent isocitrate dehydrogenase peroxisomal; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; PICD; anti-IDH1 antibody

• Host: Mouse
• Reactivity: Human.; Others not known
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: IDH1/1152
• Specificity: It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
• Form/Format: 7.0ml of Ready-to-Use for IHC staining of formalin-fixed tissues.
• Sequence Length: 414

• Applicable Applications for anti-IDH1 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cytoplasmic & Nuclear
• Immunogen: Recombinant fragment (119 Amino acid residues around aa 280-420) of human IDH1 protein
• Hu-Chromosome Location: 2q33.3
• Positive Control: HepG2, HeLa, HT29 or MCF7 cells. Breast, Colon or Prostate Carcinoma.
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with IDH1 Monoclonal Antibody (IDH1/1152).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IDH1 Monoclonal Antibody (IDH1/1152).)

References

Geisbrecht, B.V. and Gould, S.J. 1999. The human PICD gene encodes a cytoplasmic and peroxisomal NADP+-dependent isocitrate dehydrogenase. J. Biol. Chem. 274: 30527-30533

NCBI and Uniprot Product Information

• NCBI GI #: 538917488
• NCBI GeneID: 3417
• NCBI Accession #: NP_001269315.1
• NCBI GenBank Nucleotide #: NM_001282386.1
• UniProt Accession #: O75874; Q567U4; Q6FHQ6; Q7Z3V0; Q93090; Q9NTJ9; Q9UKW8
• Molecular Weight: 45-47kDa
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase (NADP(+)) 1, cytosolic
• NCBI Official Symbol: IDH1
• NCBI Official Synonym Symbols: IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• NCBI Protein Information: isocitrate dehydrogenase [NADP] cytoplasmic
• UniProt Protein Name: Isocitrate dehydrogenase [NADP] cytoplasmic
• UniProt Gene Name: IDH1
• UniProt Synonym Gene Names: PICD; IDH
• UniProt Entry Name: IDHC_HUMAN