WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using IDH3A Antibody. The lane on the left was treated with blocking peptide.)
Rabbit IDH3A Polyclonal Antibody | anti-IDH3A antibody
IDH3A Antibody
Reactivity
Human, Mouse, RatPredicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
IDH3A, Antibody; IDH3A Antibody; H IDH alpha; HIDH alpha; IDH3A; IDH3A_HUMAN; Isocitrate dehydrogenase (NAD+) alpha chain precursor; Isocitrate dehydrogenase [NAD] subunit alpha; Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial; Isocitrate dehydrogenase 3 (NAD+) alpha; Isocitrate dehydrogenase; Isocitric dehydrogenase; Isocitric dehydrogenase subunit alpha, mitochondrial; NAD; NAD(+)-specific ICDH subunit alpha; NAD(H) specific isocitrate dehydrogenase alpha subunit precursor; NAD+ specific ICDH; NADsubunit alpha mitochondrial precursor; OTTHUMP00000184906; anti-IDH3A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
IDH3A Antibody detects endogenous levels of IDH3A.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
366
Applicable Applications for anti-IDH3A antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human IDH3A.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Mitochondrion
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using IDH3A Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using IDH3A Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using IDH3A Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using IDH3A Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-IDH3A antibody
Description: Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
Subunit Structure: Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.
Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Subunit Structure: Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.
Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 40 kDa
Predicted: 40 kDa
Predicted: 40 kDa
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Synonym Full Names
isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha
NCBI Official Symbol
IDH3A
NCBI Protein Information
isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
UniProt Protein Name
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
UniProt Gene Name
IDH3A
UniProt Entry Name
IDH3A_HUMAN
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Product Notes
The IDH3A idh3a (Catalog #AAA328673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IDH3A Antibody reacts with Human, Mouse, Rat Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's IDH3A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the IDH3A idh3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IDH3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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