WB (Western Blot)
(Western blot analysis of Isocitrate dehydrogenase in MCF-7, Jurkat cell line lysates (35ug/lane). Isocitrate dehydrogenase (arrow) was detected using the purified Pab.)
Rabbit IsocitRe dehydrogenase (IDH3) Polyclonal Antibody | anti-IDH3A antibody
IsocitRe dehydrogenase (IDH3) Antibody (C-term)
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IsocitRe dehydrogenase (IDH3), Antibody; IsocitRe dehydrogenase (IDH3) Antibody (C-term); Isocitrate dehydrogenase [NAD] subunit alpha; mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; IDH3A; anti-IDH3A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Isocitrate dehydrogenase (IDH3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human Isocitrate dehydrogenase (IDH3).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
317-346
Sequence Length
366
Applicable Applications for anti-IDH3A antibody
ELISA, WB (Western Blot)
Function
Catalytic subunit of the enzyme whichcatalyzes the decarboxylation of isocitrate(ICT) into alpha-ketoglutarate. Theheterodimer composed of the alpha (IDH3A)and beta (IDH3B) subunits and theheterodimer composed of the alpha (IDH3A)and gamma (IDH3G) subunits, haveconsiderable basal activity but the full activityof the heterotetramer (containing twosubunits of IDH3A, one of IDH3B and one ofIDH3G) requires the assembly andcooperative function of both heterodimers.
Cellular Location
Mitochondrion.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of Isocitrate dehydrogenase in MCF-7, Jurkat cell line lysates (35ug/lane). Isocitrate dehydrogenase (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of Isocitrate dehydrogenase in MCF-7, Jurkat cell line lysates (35ug/lane). Isocitrate dehydrogenase (arrow) was detected using the purified Pab.)
Related Product Information for anti-IDH3A antibody
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Product Categories/Family for anti-IDH3A antibody
References
Soundar, S., et al., J. Biol. Chem. 278(52):52146-52153 (2003).
Weiss, C., et al., Biochemistry 39(7):1807-1816 (2000).
Kim, Y.O., et al., J. Biol. Chem. 274(52):36866-36875 (1999).
Huh, T.L., et al., Genomics 32(2):295-296 (1996).
Kim, Y.O., et al., Biochem. J. 308 (PT 1), 63-68 (1995) (): ().
Weiss, C., et al., Biochemistry 39(7):1807-1816 (2000).
Kim, Y.O., et al., J. Biol. Chem. 274(52):36866-36875 (1999).
Huh, T.L., et al., Genomics 32(2):295-296 (1996).
Kim, Y.O., et al., Biochem. J. 308 (PT 1), 63-68 (1995) (): ().
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39592
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Synonym Full Names
isocitrate dehydrogenase 3 (NAD+) alpha
NCBI Official Symbol
IDH3A
NCBI Protein Information
isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
UniProt Protein Name
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
UniProt Gene Name
IDH3A
UniProt Entry Name
IDH3A_HUMAN
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Product Notes
The IDH3A idh3a (Catalog #AAA289257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 317-346. The IsocitRe dehydrogenase (IDH3) Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's IsocitRe dehydrogenase (IDH3) can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IDH3A idh3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IsocitRe dehydrogenase (IDH3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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