FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28788 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human IDUA Polyclonal Antibody | anti-IDUA antibody
IDUA Antibody (Center)
IHC-P~~1:50~100
FC~~1:25
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28788 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28788 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with AAA28788 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with AAA28788 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
IHC (Immunohistchemistry)
(IDUA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IDUA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistchemistry)
(IDUA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IDUA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(All lanes : Anti-IDUA Antibody (Center) at 1:2000 dilution Lane 1: human liver lysates Lane 2: human lung lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-IDUA Antibody (Center) at 1:2000 dilution Lane 1: human liver lysates Lane 2: human lung lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-IDUA Antibody (Center) at 1:1000 dilution + human lung lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-IDUA Antibody (Center) at 1:1000 dilution + human lung lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(IDUA Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IDUA antibody detected the IDUA protein (arrow).)
WB (Western Blot)
(IDUA Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IDUA antibody detected the IDUA protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from A549 cell line, rat lung tissue lysate (from left to right), using IDUA Antibody (Center). AAA28788 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A549 cell line, rat lung tissue lysate (from left to right), using IDUA Antibody (Center). AAA28788 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Anti-IDUA Antibody (Center)at 1:2000 dilution + human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 73 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-IDUA Antibody (Center)at 1:2000 dilution + human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 73 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Vazna, A., et al. Am. J. Med. Genet. A 149A (5), 965-974 (2009)
Sugawara, K., et al. J. Hum. Genet. 53(5):467-474(2008)
NCBI and Uniprot Product Information
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Product Notes
The IDUA idua (Catalog #AAA28788) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 236-264. The IDUA Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IDUA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-P (IHC-P), Flow Cytometry (FC). WB~~1:2000 IHC-P~~1:50~100 FC~~1:25. Researchers should empirically determine the suitability of the IDUA idua for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IDUA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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