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Standard Curve (Sample)

Bovine Transforming Growth Factor Alpha ELISA Kit | TGFa elisa kit

Bovine Transforming Growth Factor Alpha ELISA Kit

Gene Names
TGFA; TFGA
Reactivity
Bovine
Synonyms
Transforming Growth Factor Alpha; N/A; Bovine Transforming Growth Factor Alpha ELISA Kit; TGFa elisa kit
Ordering
 
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For Research Use Only!
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Intended Uses
This TGF-alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine TGF-alpha. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for TGFa elisa kit
Principle of the assay: TGF-alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGF-alpha antibody and an TGF-alpha-HRP conjugate. The assay sample and buffer are incubated together with TGF-alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGF-alpha concentration since TGF-alpha from samples and TGF-alpha-HRP conjugate compete for the anti-TGF-alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by TGF-alpha from the sample, fewer sites are left to bind TGF-alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGF-alpha concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TGFa elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
17,329 Da
NCBI Official Full Name
transforming growth factor alpha, partial
NCBI Official Synonym Full Names
transforming growth factor, alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
NCBI Protein Information
protransforming growth factor alpha; TGF-alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
UniProt Entry Name
TGFA_HUMAN

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Product Notes

The Bovine TGFa tgfa (Catalog #AAA16894) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16894 ELISA Kit recognizes Bovine TGFa. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor Alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.