IHC (Immunohiostchemistry)
(AAA329055 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit IEX1 Polyclonal Antibody | anti-IER3 antibody
IEX1 Antibody
Gene Names
IER3; DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
Reactivity
HumanPredicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
IEX1, Antibody; IEX1 Antibody; DIF2; Differentiation-dependent gene 2 protein; GLY96; IER3; IEX1_HUMAN; IEX1L; Immediate early protein GLY96; Immediate early response 3; Immediate early response 3 protein; Immediately early gene X 1; PACAP-responsive gene 1 protein; PRG 1; PRG1; Protein DIF 2; Protein DIF-2; Protein PRG1; Radiation-inducible immediate-early gene IEX-1; anti-IER3 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
IEX1 Antibody detects endogenous levels of total IEX1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
156
Applicable Applications for anti-IER3 antibody
ELISA
Immunogen
A synthesized peptide derived from human IEX1, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA329055 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329055 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Human Adrenal, using IEX1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Human Adrenal, using IEX1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-IER3 antibody
Function: May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
Post Translational Modifications: Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.Glycosylated.
Subcellular Location: Membrane>Single-pass type II membrane protein.
Subunit Structure: Interacts with the PPP2R5C-PP2A holoenzyme and ERK kinases; regulates ERK dephosphorylation.
Similarity: Belongs to the IER3 family.
Post Translational Modifications: Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.Glycosylated.
Subcellular Location: Membrane>Single-pass type II membrane protein.
Subunit Structure: Interacts with the PPP2R5C-PP2A holoenzyme and ERK kinases; regulates ERK dephosphorylation.
Similarity: Belongs to the IER3 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)16kD.
Predicted Molecular Weight: (Calculated)17kDa.
Predicted Molecular Weight: (Calculated)17kDa.
NCBI Official Full Name
radiation-inducible immediate-early gene IEX-1
NCBI Official Synonym Full Names
immediate early response 3
NCBI Official Symbol
IER3
NCBI Official Synonym Symbols
DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
NCBI Protein Information
radiation-inducible immediate-early gene IEX-1
UniProt Protein Name
Radiation-inducible immediate-early gene IEX-1
UniProt Gene Name
IER3
UniProt Synonym Gene Names
DIF2; IEX1; PRG1; Protein DIF-2; Protein PRG1
UniProt Entry Name
IEX1_HUMAN
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Product Notes
The IER3 ier3 (Catalog #AAA329055) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IEX1 Antibody reacts with Human Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's IEX1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the IER3 ier3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IEX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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