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FCM/FACS (Flow Cytometry)
(IER3 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse IER3 Polyclonal Antibody | anti-IER3 antibody
IER3 Antibody (N-term)
Gene Names
IER3; DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IER3, Antibody; IER3 Antibody (N-term); Radiation-inducible immediate-early gene IEX-1; Differentiation-dependent gene 2 protein; Protein DIF-2; Immediate early protein GLY96; Immediate early response 3 protein; PACAP-responsive gene 1 protein; Protein PRG1; IER3; DIF2; IEX1; PRG1; anti-IER3 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-65 amino acids from the N-terminal region of human IER3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.36 (varies by lot)
Sequence Positions
38-65
Sequence Length
156
Applicable Applications for anti-IER3 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(IER3 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(IER3 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of IER3 Antibody (N-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of IER3 Antibody (N-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemisry)
(IER3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IER3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(IER3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IER3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(IER3 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).)
WB (Western Blot)
(IER3 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).)
WB (Western Blot)
(IER3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).)
WB (Western Blot)
(IER3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).)
Related Product Information for anti-IER3 antibody
This gene functions in the protection of cells from Fas-
or tumor necrosis factor type alpha-induced apoptosis. Partially
degraded and unspliced transcripts are found after virus infection
in vitro, but these transcripts are not found in vivo and do not
generate a valid protein.
or tumor necrosis factor type alpha-induced apoptosis. Partially
degraded and unspliced transcripts are found after virus infection
in vitro, but these transcripts are not found in vivo and do not
generate a valid protein.
Product Categories/Family for anti-IER3 antibody
References
Shahid, M., et al. Hypertension 56(4):705-712(2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009) :
Do, T.N., et al. Cancer Genet. Cytogenet. 195(1):31-36(2009)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009) :
Do, T.N., et al. Cancer Genet. Cytogenet. 195(1):31-36(2009)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16903
NCBI Official Full Name
radiation-inducible immediate-early gene IEX-1
NCBI Official Synonym Full Names
immediate early response 3
NCBI Official Symbol
IER3
NCBI Official Synonym Symbols
DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
NCBI Protein Information
radiation-inducible immediate-early gene IEX-1
UniProt Protein Name
Radiation-inducible immediate-early gene IEX-1
UniProt Gene Name
IER3
UniProt Synonym Gene Names
DIF2; IEX1; PRG1; Protein DIF-2; Protein PRG1
UniProt Entry Name
IEX1_HUMAN
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Product Notes
The IER3 ier3 (Catalog #AAA287626) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 38-65. The IER3 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IER3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IER3 ier3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IER3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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