IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit IFNAR1 Polyclonal Antibody | anti-IFNAR1 antibody
IFNAR1 Rabbit pAb
IHC: 1:50-1:200
IF: 1:50-1:200
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using IFNAR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using IFNAR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)
NCBI and Uniprot Product Information
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Product Notes
The IFNAR1 ifnar1 (Catalog #AAA28371) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFNAR1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IFNAR1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the IFNAR1 ifnar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFNAR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What cell types or tissues express IFNAR1 and can be detected with this antibody?
IFNAR1 (IFN-α/β receptor 1) is expressed on nearly all cell types, including lymphocytes, monocytes, macrophages, dendritic cells, fibroblasts, endothelial cells, and epithelial cells. This ubiquitous expression enables detection across diverse tissues, making this rabbit polyclonal antibody versatile for studying Type I interferon responses across virtually any tissue or cell type.
Can the IFNAR1 rabbit pAb be used for flow cytometry or immunofluorescence?
Yes, this rabbit polyclonal antibody is validated for both flow cytometry (enabling IFNAR1+ cell identification and sorting) and immunofluorescence (IF) applications for subcellular localization in fixed cells and tissues. These applications facilitate comprehensive characterization of IFNAR1 expression patterns and cellular responses to interferon stimulation.
Is IFNAR1 expression upregulated after interferon stimulation?
Yes, IFNAR1 expression is dynamically upregulated following Type I interferon (IFN-α/β) stimulation or viral infection, typically within hours. This upregulation amplifies the interferon signaling response and represents a positive feedback mechanism where IFNAR1 surface levels increase to enhance cellular sensitivity to circulating Type I interferons.
Can this IFNAR1 antibody detect endogenous levels of the receptor in human samples?
Yes, this rabbit polyclonal antibody specifically recognizes endogenous human IFNAR1 protein. It detects both cell surface and intracellular IFNAR1 pools, enabling assessment of basal expression, interferon-induced upregulation, and receptor internalization/trafficking in human samples from various tissues and cell types.
Does fixation method affect IFNAR1 staining quality?
Yes, fixation method significantly impacts IFNAR1 staining quality in immunofluorescence assays. Standard paraformaldehyde (4%) fixation generally preserves IFNAR1 antigenicity well, but prolonged fixation or cross-linking may reduce epitope accessibility. Using appropriate fixation times and mild permeabilization optimizes staining quality and specificity.
Is this IFNAR1 antibody suitable for Western blot analysis?
Yes, this rabbit polyclonal antibody is suitable for Western blot analysis, enabling detection of IFNAR1 (~110 kDa) in cell lysates and tissue homogenates. Western blotting allows quantitative assessment of IFNAR1 protein levels across samples and conditions, including detection of phosphorylated IFNAR1 forms in activated signaling states.
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