IF (Immunofluorescence)
(Immunofluorescence staining of Nfkbib in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- Nfkbib polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to cytoplasm and nucleus.)
Rabbit anti-Mouse IKB beta/NFKBIB Polyclonal Antibody | anti-NFKBIB antibody
Anti-IKB beta/NFKBIB Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
IKB beta/NFKBIB, Antibody; Anti-IKB beta/NFKBIB Antibody, Rabbit Polyclonal; NFKBIB/IkB-beta Antibody, Rabbit PAb, Antigen Affinity Purified; NFKB inhibitor beta; Anti-I(Kappa)B(beta) Antibody; Anti-I-kappa-B-beta Antibody; Anti-IKappaBbeta Antibody; Anti-IkB Antibody; Anti-ikB-B Antibody; Anti-IKB-beta Antibody; Anti-IkBb Antibody; Anti-NF-kappa-BIB Antibody; anti-NFKBIB antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse NFKBIB/IkB-beta
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NFKBIB antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Mouse NFKBIB/IkB-beta
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse NFKBIB/IkB-beta, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of Nfkbib in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- Nfkbib polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of Nfkbib in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- Nfkbib polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to cytoplasm and nucleus.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human Nfkbib in human breast carcinoma with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human Nfkbib in human breast carcinoma with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)
WB (Western Blot)
(Anti-Nfkbib rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: THP-1 Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:31 kDa)
WB (Western Blot)
(Anti-Nfkbib rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: THP-1 Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:31 kDa)
Related Product Information for anti-NFKBIB antibody
NFKBIB (NFKB Inhibitor Beta) is a Protein Coding gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. NF-kappaB regulation involves the inhibitor protein NFKBIB, which form complexes with NF-kappaB to sequester it in the cytoplasm. Overexpression of NFKBIB protein in IAV infected cells led to lower levels of IAV. MiR-20a could promote activation of the NFkappaB pathway and downstream targets Livin and Survivin by targeting NFKBIB, which potentially contributed to GC chemoresistance.
Product Categories/Family for anti-NFKBIB antibody
NCBI and Uniprot Product Information
UniProt Accession #
UniProt Protein Name
NF-kappa-B inhibitor beta
UniProt Gene Name
Nfkbib
UniProt Synonym Gene Names
Ikbb
UniProt Entry Name
IKBB_MOUSE
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Product Notes
The NFKBIB nfkbib (Catalog #AAA254668) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IKB beta/NFKBIB Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IKB beta/NFKBIB can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NFKBIB nfkbib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKB beta/NFKBIB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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