WB (Western Blot)
(Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) antibody.The lane on the right is treated with the antigen-specific peptide.)
Rabbit IKK-alpha/ beta Polyclonal Antibody | anti-IKK-alpha/ beta antibody
IKK-alpha/ beta (Phospho-Ser176/177) Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Synonyms
IKK-alpha/ beta, Antibody; IKK-alpha/ beta (Phospho-Ser176/177) Antibody; FLJ40509; I-kappa-B kinase; IKBKB; kinase beta; NFKBIKB; anti-IKK-alpha/ beta antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
745
Applicable Applications for anti-IKK-alpha/ beta antibody
WB (Western Blot)
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK-alpha/beta.
Modification
Phospho-Ser176/177
Target Name
IKK- alpha/ beta
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) antibody.The lane on the right is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) antibody.The lane on the right is treated with the antigen-specific peptide.)
Related Product Information for anti-IKK-alpha/ beta antibody
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Chandrakesan P, et al. (2010)J Biol Chem 285, 33485-98Hinz M, et al. (2010£© Mol Cell 40, 63-74 Choudhary S, Lu M, Cui R, Brasier AR (2007)Mol Endocrinol 21, 2203-17
Product Categories/Family for anti-IKK-alpha/ beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
SDS-PAGE MW: 85kDa
NCBI Official Full Name
inhibitor of nuclear factor kappa-B kinase subunit alpha
NCBI Official Synonym Full Names
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol
CHUK
NCBI Official Synonym Symbols
IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit alpha
UniProt Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
UniProt Gene Name
CHUK
UniProt Synonym Gene Names
IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16
UniProt Entry Name
IKKA_HUMAN
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Product Notes
The IKK-alpha/ beta chuk (Catalog #AAA308010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IKK-alpha/ beta (Phospho-Ser176/177) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IKK-alpha/ beta can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the IKK-alpha/ beta chuk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKK-alpha/ beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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