WB (Western Blot)
(Western blot analysis of IL1F6 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse IL1F6 Polyclonal Antibody | anti-IL1F6 antibody
IL1F6 Antibody
Gene Names
IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IL1F6, Antibody; IL1F6 Antibody; FIL1; FIL1 epsilon; FIL1(EPSILON); FIL1E; IL 1 epsilon; IL 1F6; IL 1H1; IL-1 epsilon; IL-1F6; IL1E; IL1F6; IL1F6_HUMAN; IL1RP2; IL36A; Interleukin 1 epsilon; Interleukin 1 family member 6 (epsilon); Interleukin 1 family member 6; Interleukin 36 alpha; Interleukin-1 epsilon; Interleukin-1 family member 6; MGC129552; MGC129553; MGC151479; MGC151481; OTTMUSP00000012798; RP23-176J12.4; anti-IL1F6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
IL1F6 antibody detects endogenous levels of total IL1F6.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
158
Applicable Applications for anti-IL1F6 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human IL1F6, corresponding to a region within C terminal amino acids.
Preparation and Storage
Store at -20°C.
WB (Western Blot)
(Western blot analysis of IL1F6 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of IL1F6 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-IL1F6 antibody
Function: Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes upregulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by upregulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. May play a role in proinflammatory effects in the lung.
Post-translational Modifications: N-terminal truncation leads to a dramatic enhancement of its activity (> 1000-fold).
Similarity: Belongs to the IL-1 family.
Post-translational Modifications: N-terminal truncation leads to a dramatic enhancement of its activity (> 1000-fold).
Similarity: Belongs to the IL-1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25kDa
NCBI Official Full Name
interleukin-36 alpha
NCBI Official Synonym Full Names
interleukin 36 alpha
NCBI Official Symbol
IL36A
NCBI Official Synonym Symbols
FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
NCBI Protein Information
interleukin-36 alpha
UniProt Protein Name
Interleukin-36 alpha
UniProt Gene Name
IL36A
UniProt Synonym Gene Names
FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6
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Product Notes
The IL1F6 il36a (Catalog #AAA323463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL1F6 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL1F6 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IL1F6 il36a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL1F6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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