Rabbit anti-Human IL1F6 Polyclonal Antibody | anti-IL36A antibody

IL1F6 Antibody (Center)

Cat. Num. AAA290310

• Gene Names: IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: IL1F6, Antibody; IL1F6 Antibody (Center); Interleukin-36 alpha; FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6; anti-IL36A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This IL1F6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-61 amino acids from the Central region of human IL1F6.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 35-61
• Sequence Length: 158

• Applicable Applications for anti-IL36A antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes upregulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by upregulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. May play a role in proinflammatory effects in the lung.
• Cellular Location: Cytoplasm. Secreted. Note=The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion.
• Tissue Location: Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines Predominantly expressed in skin
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(IL1F6 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(IL1F6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL1F6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of IL1F6 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).IL1F6 (arrow) was detected using the purified Pab.)

Related Product Information for anti-IL36A antibody

The function of this protein has not been specifically defined.

Product Categories/Family for anti-IL36A antibody

Immunology

References

Kim, T.J., et al. J. Rheumatol. 35(8):1603-1608(2008)
Blumberg, H., et al. J. Exp. Med. 204(11):2603-2614(2007)
Zhan, X., et al. Anal. Biochem. 354(2):279-289(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 7657092
• NCBI GeneID: 27179
• NCBI Accession #: NP_055255.1
• NCBI GenBank Nucleotide #: NM_014440.1
• UniProt Accession #: Q9UHA7; Q53SR7; Q5BLR4; Q7RTZ8; B2RAD9
• Molecular Weight: 17684
• NCBI Official Full Name: interleukin-36 alpha
• NCBI Official Synonym Full Names: interleukin 36, alpha
• NCBI Official Symbol: IL36A
• NCBI Official Synonym Symbols: FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
• NCBI Protein Information: interleukin-36 alpha
• UniProt Protein Name: Interleukin-36 alpha
• UniProt Gene Name: IL36A
• UniProt Synonym Gene Names: FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6
• UniProt Entry Name: IL36A_HUMAN