FCM/FACS (Flow Cytometry)
(Dilution: Blank control:TM4(blue). Primary Antibody: Rabbit Anti-IL1RAPL1 antibody(bs-0445R), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Protocol Primary antibody (bs-0445R,1 ug /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.)
Rabbit IL1RAPL1 Polyclonal Antibody | anti-IL1RAPL1 antibody
IL1RAPL1 Polyclonal Antibody
Gene Names
IL1RAPL1; IL1R8; MRX10; MRX21; MRX34; OPHN4; IL1RAPL; TIGIRR-2
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IL1RAPL1, Antibody; IL1RAPL1 Polyclonal Antibody; Interleukin-1 receptor accessory protein-like 1; IL-1-RAPL-1; IL-1RAPL-1; IL1RAPL-1; 3.2.2.6; Oligophrenin-4; Three immunoglobulin domain-containing IL-1 receptor-related 2; TIGIRR-2; X-linked interleukin-1 receptor accessory protein-like 1; IL1RAPL1; OPHN4; anti-IL1RAPL1 antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-IL1RAPL1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Tissue Location
Detected at low levels in heart, skeletal muscle, ovary, skin, amygdala, caudate nucleus, corpus callosum, hippocampus, substantia nigra and thalamus. Detected at very low levels in tonsil, prostate, testis, small intestine, placenta, colon and fetal liver.
Notes
ECO:0000255|PROSITE-ProRule: PRU00204
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Dilution: Blank control:TM4(blue). Primary Antibody: Rabbit Anti-IL1RAPL1 antibody(bs-0445R), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Protocol Primary antibody (bs-0445R,1 ug /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Dilution: Blank control:TM4(blue). Primary Antibody: Rabbit Anti-IL1RAPL1 antibody(bs-0445R), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Protocol Primary antibody (bs-0445R,1 ug /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.)
IHC (Immunohiostchemistry)
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-IL1RAPL1 Polyclonal Antibody, Unconjugated(bs-0445R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohiostchemistry)
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-IL1RAPL1 Polyclonal Antibody, Unconjugated(bs-0445R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
WB (Western Blot)
(Sample:Cerebrum (Mouse) Lysate at 40 ugHippocampal (Mouse) Lysate at 40 ugPrimary: Anti-IL1RAPL1 (bs-0445R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 108 kD)
WB (Western Blot)
(Sample:Cerebrum (Mouse) Lysate at 40 ugHippocampal (Mouse) Lysate at 40 ugPrimary: Anti-IL1RAPL1 (bs-0445R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 108 kD)
Related Product Information for anti-IL1RAPL1 antibody
May regulate secretion and presynaptic differentiation through inhibition of the activity of N-type voltage-gated calcium channel (PubMed:12783849). May activate the MAP kinase JNK (PubMed:15123616). Plays a role in neurite outgrowth (By similarity). During dendritic spine formation can bidirectionally induce pre- and post-synaptic differentiation of neurons by trans-synaptically binding to PTPRD (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
696
NCBI Official Full Name
Interleukin-1 receptor accessory protein-like 1
NCBI Official Synonym Full Names
interleukin 1 receptor accessory protein-like 1
NCBI Official Symbol
IL1RAPL1
NCBI Official Synonym Symbols
IL1R8; MRX10; MRX21; MRX34; OPHN4; IL1RAPL; TIGIRR-2
NCBI Protein Information
interleukin-1 receptor accessory protein-like 1; IL1RAPL-1; IL-1RAPL-1; IL-1-RAPL-1; oligophrenin-4; interleukin 1 receptor-8; mental retardation, X-linked 10; X-linked interleukin-1 receptor accessory protein-like 1; three immunoglobulin domain-containin
UniProt Protein Name
Interleukin-1 receptor accessory protein-like 1
UniProt Gene Name
IL1RAPL1
UniProt Synonym Gene Names
OPHN4; IL-1-RAPL-1; IL-1RAPL-1; IL1RAPL-1; TIGIRR-2
UniProt Entry Name
IRPL1_HUMAN
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Product Notes
The IL1RAPL1 il1rapl1 (Catalog #AAA291266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL1RAPL1 Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's IL1RAPL1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IL1RAPL1 il1rapl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL1RAPL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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