WB (Western Blot)
(Anti- IL-3 antibody, Western blottingAll lanes: Anti IL-3 at 0.5ug/mlLane 1: PC-12 Whole Cell Lysate at 40ugLane 2: NRK Whole Cell Lysate at 40ugLane 3: RH35 Whole Cell Lysate at 40ugPredicted bind size: 17KDObserved bind size: 37KD)
anti-Rat IL3 Polyclonal Antibody | anti-IL3 antibody
Anti-IL3 Antibody
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
IL3, Antibody; Anti-IL3 Antibody; Interleukin-3; Colony stimulating factor multiple; Hematopoietic growth factor; IL 3; IL-3; IL3; IL3_HUMAN; Interleukin 3 (colony stimulating factor, multiple); Interleukin 3; Mast cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; Multipotential colony stimulating factor; Multipotential colony-stimulating factor; OTTHUMP00000065963; P cell stimulating factor; P-cell-stimulating factor; interleukin 3; anti-IL3 antibody
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
166
Applicable Applications for anti-IL3 antibody
ELISA, WB (Western Blot)
Immunogen
E Coli-derived rat IL3 recombinant protein (Position: S28-C166). Rat IL3 shares 32% and 55% amino acid (aa) sequence identity with human and mouse IL3, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti- IL-3 antibody, Western blottingAll lanes: Anti IL-3 at 0.5ug/mlLane 1: PC-12 Whole Cell Lysate at 40ugLane 2: NRK Whole Cell Lysate at 40ugLane 3: RH35 Whole Cell Lysate at 40ugPredicted bind size: 17KDObserved bind size: 37KD)
WB (Western Blot)
(Anti- IL-3 antibody, Western blottingAll lanes: Anti IL-3 at 0.5ug/mlLane 1: PC-12 Whole Cell Lysate at 40ugLane 2: NRK Whole Cell Lysate at 40ugLane 3: RH35 Whole Cell Lysate at 40ugPredicted bind size: 17KDObserved bind size: 37KD)
WB (Western Blot)
(Anti- IL-3 antibody, Western blottingAll lanes: Anti IL-3 at 0.5ug/mlWB: Recombinant Rat IL-3 Protein 0.5ngPredicted bind size: 15KDObserved bind size: 15KD)
WB (Western Blot)
(Anti- IL-3 antibody, Western blottingAll lanes: Anti IL-3 at 0.5ug/mlWB: Recombinant Rat IL-3 Protein 0.5ngPredicted bind size: 15KDObserved bind size: 15KD)
Related Product Information for anti-IL3 antibody
Description: Rabbit IgG polyclonal antibody for Interleukin-3(IL3) detection. Tested with WB, ELISA in Rat.
Background: Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
Background: Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
References
1. Yang YC, Ciarletta AB, Temple PA, Chung MP, Kovacic S, Witek-Giannotti JS, Leary AC, Kriz R, Donahue RE, Wong GG (October 1986). "Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell 47 (1): 3-10. 2. Webb, G. C., Lee, J. S., Campbell, H. D., Young, I. G. The genes for interleukins 3 and 5 map to the same locus on mouse chromosome 11. Cytogenet. Cell Genet. 50: 107-110, 1989.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,631 Da
NCBI Official Full Name
Interleukin-3
NCBI Official Synonym Full Names
interleukin 3
NCBI Official Symbol
Il3
NCBI Protein Information
interleukin-3
UniProt Protein Name
Interleukin-3
UniProt Gene Name
Il3
UniProt Synonym Gene Names
Il-3; IL-3; MCGF
UniProt Entry Name
IL3_RAT
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Product Notes
The IL3 il3 (Catalog #AAA46584) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL3 Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL3 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IL3 il3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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