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IHC (Immunohistochemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Rabbit ILK Polyclonal Antibody | anti-ILK antibody
Phospho-ILK (Ser343) Antibody
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (89%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
ILK, Antibody; Phospho-ILK (Ser343) Antibody; 59kDa serine/threonine protein kinase; 59kDa serine/threonine-protein kinase; DKFZp686F1765; Epididymis secretory protein Li 28; HEL S 28; ILK 1; ILK 2; ILK; ILK-1; ILK-2; ILK_HUMAN; ILK1; ILK2; Integrin linked kinase 2; Integrin linked Kinase; Integrin linked protein kinase; Integrin-linked protein kinase; p59; p59ILK; anti-ILK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (89%)
Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (89%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-ILK (Ser343) Antibody detects endogenous levels of ILK only when phosphorylated at Ser343.
Tissue Specificity: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Tissue Specificity: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ILK antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ILK around the phosphorylation site of Ser343.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Autophosphorylated on serine residues.
Subunit Structure
Interacts with FERMT2 (By similarity). Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB; these compete for the same binding site. Interacts probably also with TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts with LIMD2; leading to activate the protein kinase activity.
Similarity
A PH-like domain is involved in phosphatidylinositol phosphate binding.Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Subcellular Location
Cell junction>Focal adhesion. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium. Cytoplasm>Myofibril>Sarcomere.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis ILK-1 (Phospho-Ser343) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis ILK-1 (Phospho-Ser343) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of extracts from Mouse heart, using Phospho-ILK-1 (Ser343) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse heart, using Phospho-ILK-1 (Ser343) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-ILK antibody
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,419 Da
NCBI Official Full Name
integrin-linked protein kinase isoform 1
NCBI Official Synonym Full Names
integrin-linked kinase
NCBI Official Symbol
ILK
NCBI Official Synonym Symbols
P59; ILK-1; ILK-2; p59ILK
NCBI Protein Information
integrin-linked protein kinase; integrin-linked kinase-2; 59 kDa serine/threonine-protein kinase
UniProt Protein Name
Integrin-linked protein kinase
UniProt Gene Name
ILK
UniProt Synonym Gene Names
ILK1; ILK2
UniProt Entry Name
ILK_HUMAN
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Product Notes
The ILK ilk (Catalog #AAA329663) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ILK (Ser343) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (89%) and may cross-react with other species as described in the data sheet. AAA Biotech's ILK can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ILK ilk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ILK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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