WB (Western Blot)
(Anti-IDO1 antibody, AAA44953, Western blottingAll lanes: Anti IDO1 (AAA44953) at 0.5ug/mlLane 1: SMMC Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: 293T Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD)
Rabbit Indoleamine 2, 3-dioxygenase Polyclonal Antibody | anti-IDO1 antibody
Anti-Indoleamine 2, 3-dioxygenase antibody
Gene Names
IDO1; IDO; INDO; IDO-1
Reactivity
Reacts with: Human
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Indoleamine 2, 3-dioxygenase, Antibody; Anti-Indoleamine 2, 3-dioxygenase antibody; Indoleamine 2,3-dioxygenase 1(IDO-1); indoleamine 2,3-dioxygenase 1; 3-dioxygenase antibody; I23O1_HUMAN antibody; IDO 1 antibody; IDO antibody; IDO-1 antibody; IDO1 antibody; INDO antibody; indolamine 2,3 dioxygenase antibody; Indole 2 3 dioxygenase antibody; indoleamine 2 3 dioxygenase 1 antibody; indoleamine 2 3 dioxygenase antibody; Indoleamine 2,3-dioxygenase 1 antibody; Indoleamine pyrrole 2 3 dioxygenase antibody; Indoleamine-pyrrole 2 antibody; anti-IDO1 antibody
Host
Rabbit
Reactivity
Reacts with: Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
403
Applicable Applications for anti-IDO1 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IDO1(1-20aa MAHAMENSWTISKEYHIDEE).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml
Buffer Solution
Phosphate buffered saline
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-IDO1 antibody, AAA44953, Western blottingAll lanes: Anti IDO1 (AAA44953) at 0.5ug/mlLane 1: SMMC Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: 293T Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD)
WB (Western Blot)
(Anti-IDO1 antibody, AAA44953, Western blottingAll lanes: Anti IDO1 (AAA44953) at 0.5ug/mlLane 1: SMMC Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: 293T Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD)
Related Product Information for anti-IDO1 antibody
Description: Rabbit IgG polyclonal antibody for Indoleamine 2,3-dioxygenase 1(IDO1) detection. Tested with WB in Human.
Background: IDO1(INDOLEAMINE 2,3-DIOXYGENASE), INDO or IDO, is an immunomodulatory enzyme produced by some alternatively activated macrophages and other immunoregulatory cells. This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. By fluorescence in situ hybridization, the assignment is narrowed to chromosome 8p12-p11. INDO Interferon-gamma has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase. During inflammation, IDO is upregulated in dendritic cells and phagocytes by proinflammatory stimuli, most notably IFNG, and the enzyme then uses superoxide as a 'cofactor' for oxidative cleavage of the indole ring of tryptophan, yielding an intermediate that deformylates to L-kynurenine.
Background: IDO1(INDOLEAMINE 2,3-DIOXYGENASE), INDO or IDO, is an immunomodulatory enzyme produced by some alternatively activated macrophages and other immunoregulatory cells. This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. By fluorescence in situ hybridization, the assignment is narrowed to chromosome 8p12-p11. INDO Interferon-gamma has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase. During inflammation, IDO is upregulated in dendritic cells and phagocytes by proinflammatory stimuli, most notably IFNG, and the enzyme then uses superoxide as a 'cofactor' for oxidative cleavage of the indole ring of tryptophan, yielding an intermediate that deformylates to L-kynurenine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
45,326 Da
NCBI Official Full Name
Indoleamine 2,3-dioxygenase 1
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol
IDO1
NCBI Official Synonym Symbols
IDO; INDO; IDO-1
NCBI Protein Information
indoleamine 2,3-dioxygenase 1; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase
UniProt Protein Name
Indoleamine 2,3-dioxygenase 1
UniProt Gene Name
IDO1
UniProt Synonym Gene Names
IDO; INDO; IDO-1
UniProt Entry Name
I23O1_HUMAN
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Product Notes
The IDO1 ido1 (Catalog #AAA44953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Indoleamine 2, 3-dioxygenase antibody reacts with Reacts with: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Indoleamine 2, 3-dioxygenase can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the IDO1 ido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Indoleamine 2, 3-dioxygenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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