Application Data
Rabbit Integrin b4 (Tyr-1494) Polyclonal Antibody
Integrin beta4 (Tyr-1494), phospho-specific
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Integrin b4 (Tyr-1494), Antibody; Integrin beta4 (Tyr-1494), phospho-specific; Integrin b4 (Tyr-1494); phospho-specific; CD104; GP150; anti-Integrin b4 (Tyr-1494) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phosphointegrin- b4 (Tyr-1494) peptide (without carrier). The antibody detects a 200kDa* protein corresponding to the molecular mass of integrin-b4 on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but not in control cells. Similar results were also observed in src-transformed Hct116 cells treated with pervanadate.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Integrin b4 (Tyr-1494) antibody
WB (Western Blot), ELISA
Immunogen
Phospho-integrin-b4 (Tyr-1494) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human
integrin-b4. This peptide sequence is found in all three integrin-b4 isoforms and is highly conserved in rat and mouse integrin-b4.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Application Data
Application Data
Related Product Information for anti-Integrin b4 (Tyr-1494) antibody
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin alpha-6-beta-4receptors are found in basement membrane along with laminin-5. These receptors are expressed in epithelial, schwann, endothelial and some immune cells. The cytoplasmic domain of the integrin-beta-4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly this site is critical for integrin alpha-6-beta-4 increases in carcinoma invasion.
References
• Shaw, L.M. (2001). Mol Cell Biol. 21(15):5082-5093.
• Dans, M. et al. (2001). J Biol Chem. 276(2):1494-1502.
• Kim, H.I. et al. (2008) Cancer Prev Res. 1(5):385. (WB: Human b4 transfectants)
• Dans, M. et al. (2001). J Biol Chem. 276(2):1494-1502.
• Kim, H.I. et al. (2008) Cancer Prev Res. 1(5):385. (WB: Human b4 transfectants)
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Product Notes
The Integrin b4 (Tyr-1494) (Catalog #AAA71523) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Integrin beta4 (Tyr-1494), phospho-specific reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin b4 (Tyr-1494) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the Integrin b4 (Tyr-1494) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin b4 (Tyr-1494), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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