WB (Western Blot)
(Detection of human and mouse Intersectin-1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Intersectin-1 antibody AAA212108 (lot AAA212108-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse Intersectin 1 Polyclonal Antibody | anti-ITSN1 antibody
Rabbit anti-Intersectin 1 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Intersectin 1, Antibody; Rabbit anti-Intersectin 1 Antibody, Affinity Purified; intersectin-1; Src homology 3 domain-containing protein; SH3P17; SH3D1A; ITSN; intersectin short variant 12; intersectin 1 short form variant, 11; intersectin 1 short form variant 3; intersectin 1 (SH3 domain protein); human intersectin-SH3 domain-containing protein SH3P17; SH3 domain-containing protein 1A; Intersectin-1; intersectin 1; anti-ITSN1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ITSN1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 850 and 900
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Intersectin-1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Intersectin-1 antibody AAA212108 (lot AAA212108-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse Intersectin-1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Intersectin-1 antibody AAA212108 (lot AAA212108-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Intersectin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Intersectin-1 antibody AAA212108 (lot AAA212108-2) used for IP at 6 ug per reaction. Intersectin-1 was also immunoprecipitated by a previous lot of this antibody (lot AAA212108-1). For blotting immunoprecipitated Intersectin-1, AAA212108 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Intersectin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Intersectin-1 antibody AAA212108 (lot AAA212108-2) used for IP at 6 ug per reaction. Intersectin-1 was also immunoprecipitated by a previous lot of this antibody (lot AAA212108-1). For blotting immunoprecipitated Intersectin-1, AAA212108 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-ITSN1 antibody
Intersectin 1 is an adapter protein that may provide an indirect link between the endocytic membrane traffic and the actin assembly machinery. Intersectin 1 may regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15811].
Product Categories/Family for anti-ITSN1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ITSN1 itsn1 (Catalog #AAA212108) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Intersectin 1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Intersectin 1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ITSN1 itsn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Intersectin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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