FCM/FACS (Flow Cytometry)
( Flow-cytometry using the anti-CD8 beta antibody YTS 156.7.7 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTS 156.7.7 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
CD8 beta recombinant antibody
Anti-CD8 beta [YTS 156.7.7]
Gene Names
CD8B; CD8B1; CD8BP
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
PurifiedProtein A Affinity Purified
Synonyms
CD8 beta, Recombinant Antibody; Anti-CD8 beta [YTS 156.7.7]; T-cell surface glycoprotein CD8 beta chain; Lymphocyte antigen 3; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein Lyt-3; CD8b; Cd8b; Cd8b1; Ly-3; Lyt-3; Lyt3; CD8 beta recombinant antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
YTS 156.7.7
Specificity
The antibody Recognizes the B epitope of the mouse Lyt-3 (CD8) antigen. Has been usedin CD8 subset depletion in vivo and flow cytometry studies.
Purity/Purification
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Sequence Length
207
Applicable Applications for CD8 beta recombinant antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Immunogen
Mouse CD8 (lyt-3).
Antigen
CD8 beta
Antigen Description
Mouse CD8 (lyt-3).
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
Publication Note
Describes the production of monoclonal antibody to lyt-3 (CD-8) and demonstratesits effectiveness in CD8 subset depletion in mice.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
( Flow-cytometry using the anti-CD8 beta antibody YTS 156.7.7 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTS 156.7.7 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
( Flow-cytometry using the anti-CD8 beta antibody YTS 156.7.7 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTS 156.7.7 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
( Flow-cytometry using anti-CD8beta antibody YTS 156.7.7 Mouse lymphocytes were stained with an anti-CD44 control or the rabbit-chimeric version of YTS 156.7.7 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
( Flow-cytometry using anti-CD8beta antibody YTS 156.7.7 Mouse lymphocytes were stained with an anti-CD44 control or the rabbit-chimeric version of YTS 156.7.7 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Related Product Information for CD8 beta recombinant antibody
Recombinant monoclonal antibody to CD8 beta. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the hybridoma YTS 156.7.7.
Product Categories/Family for CD8 beta recombinant antibody
References
Cobbold S, Martin G, Waldmann H. Transplantation. 1986 Sep;42(3):239-47. PMID:3529524
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
23,744 Da
NCBI Official Full Name
CD8 beta
NCBI Official Synonym Full Names
CD8b molecule
NCBI Official Symbol
CD8B
NCBI Official Synonym Symbols
CD8B1; CD8BP
NCBI Protein Information
T-cell surface glycoprotein CD8 beta chain
UniProt Protein Name
CD8 beta
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Product Notes
The CD8 beta (Catalog #AAA71974) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD8 beta [YTS 156.7.7] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 beta can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD8 beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 beta, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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