WB (Western Blot)
(Detection of human IST1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IST1 antibody AAA213251 (lot AAA213251-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human IST1/OLC1 Polyclonal Antibody | anti-IST1/OLC1 antibody
Rabbit anti-IST1/OLC1 Antibody, Affinity Purified
Gene Names
IST1; OLC1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
IST1/OLC1, Antibody; Rabbit anti-IST1/OLC1 Antibody, Affinity Purified; IST1 homolog; putative MAPK-activating protein PM28; overexpressed in lung cancer 1; IST1, endosomal sorting complex required for transport-III component; increased sodium tolerance 1 homolog; hIST1; OLC1; IST1, ESCRT-III associated factor; anti-IST1/OLC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-IST1/OLC1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 300 and 350
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human IST1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IST1 antibody AAA213251 (lot AAA213251-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human IST1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IST1 antibody AAA213251 (lot AAA213251-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human IST1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IST1 antibody AAA213251 (lot AAA213251-1) used for IP at 6 ug per reaction. IST1 was also immunoprecipitated by rabbit anti-IST1 antibody BL21420. For blotting immunoprecipitated IST1, AAA213251 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human IST1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IST1 antibody AAA213251 (lot AAA213251-1) used for IP at 6 ug per reaction. IST1 was also immunoprecipitated by rabbit anti-IST1 antibody BL21420. For blotting immunoprecipitated IST1, AAA213251 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-IST1/OLC1 antibody
Increased sodium tolerance 1 homolog (IST1) is a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis [taken from NCBI Entrez Gene (Gene ID: 9798)].
Product Categories/Family for anti-IST1/OLC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,089 Da
NCBI Official Full Name
IST1 homolog isoform b
NCBI Official Synonym Full Names
increased sodium tolerance 1 homolog (yeast)
NCBI Official Symbol
IST1
NCBI Official Synonym Symbols
OLC1
NCBI Protein Information
IST1 homolog; overexpressed in lung cancer 1; putative MAPK-activating protein PM28
UniProt Protein Name
IST1 homolog
UniProt Gene Name
IST1
UniProt Synonym Gene Names
KIAA0174; hIST1
UniProt Entry Name
IST1_HUMAN
Similar Products
Product Notes
The IST1/OLC1 ist1 (Catalog #AAA213251) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-IST1/OLC1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IST1/OLC1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IST1/OLC1 ist1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IST1/OLC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.